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AMB Express
Fig. 6 | AMB Express

Fig. 6

From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Fig. 6

SDS-PAGE of cultures of E. coli BL21DE3 hosting d-Hyd(co) and d-Hyd after screening for cultivation conditions. a d-Hyd(co) insoluble fraction, 1 +IPTG −EtOH pH 7, 2 +IPTG −EtOH pH 6, 3 +IPTG +EtOH pH 7, 4 protein standard with molecular weights in kDa, 5 +IPTG +EtOH pH 6, 6 −IPTG −EtOH pH 7, 7 −IPTG −EtOH pH 6, 8 −IPTG +EtOH pH 7, 9 −IPTG +EtOH pH 6, 10 soluble fraction. b d-Hyd insoluble fraction, 1 protein standard with molecular weights in kDa, 2 +IPTG −EtOH pH 7, 3 +IPTG −EtOH pH 6, 4 +IPTG +EtOH pH 7, 5 +IPTG +EtOH pH 6, 6 −IPTG −EtOH pH 7, 7 −IPTG −EtOH pH 6, 8 −IPTG +EtOH pH 7, 9 −IPTG +EtOH pH 6, 10 soluble fraction

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