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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Fig. 3

SDS-PAGE of E. coli BL21DE3 cultures hosting d-Hyd and d-Hyd(co) after cultivation and expression under oxygen deficiency. a Qualitative analysis of the insoluble fraction, 1 protein standard with molecular weights in kDa, 2 d-Hyd at 120 rpm, 3 d-Hyd at 100 rpm, 4 d-Hyd at 90 rpm, 5 d-Hyd(co) at 120 rpm, 6 d-Hyd(co) at 100 rpm, 7 d-Hyd(co) at 90 rpm. b Qualitative analysis of the soluble fraction, 1 protein standard with molecular weights in kDa, 2 d-Hyd at 120 rpm, 3 d-Hyd at 100 rpm, 4 d-Hyd at 90 rpm, 5 d-Hyd(co) at 120 rpm, 6 d-Hyd(co) at 100 rpm, 7 d-Hyd(co) at 90 rpm

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