Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli

AMB Express

Table 3 Effects of yhaM overexpression on the production of cysteine

Strain	Plasmid	Cysteine (mg L⁻¹)	Yield (%)	OD₆₆₀
MG1655 (WT)	pMIV-cysE5, pACYC177	74.6 ± 7.0	0.187 ± 0.018	21.7 ± 0.8
MG1655 (WT)	pMIV-cysE5, pACYC-yhaM1	14.9 ± 1.1	0.037 ± 0.003	22.0 ± 0.8

Values represent the averages based on five independent experiments and the error value represents one standard deviation. To retain the plasmids, 20 μg mL⁻¹ kanamycin and 25 μg mL⁻¹ chloramphenicol were added. Cultivation was terminated at 21–24 h after inoculation when all the sugar (40 g L⁻¹) had been consumed