Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

AMB Express

Table 2 Comparison of biomass yield, DHA content, DHA productivity, lipid and DHA yield of C. cohnii under different concentrations of NaNO₃

NaNO₃ conc.	Time	Y ^a_DCW (g/g GLC)	P_L (g/L day)	Y ^a_L (mg/g GLC)	C_DHA (g/L)	P_DHA (g/L day)	Y ^a_DHA (mg/g GLC)
0.2 g/L	Growth	0.46 ± 0.032	0.38 ± 0.009	54.05 ± 3.1	0.13 ± 0.004	0.064 ± 0.000	9.01 ± 0.09
	Lipid accumulation	0.47 ± 0.05	0.48 ± 0.01	126.76 ± 17.9	0.91 ± 0.008	0.18 ± 0.001	47.83 ± 2.19
	Lipid turnover	0.43 ± 0.063	0.28 ± 0.02	95.54 ± 14.9	0.78 ± 0.009	0.11 ± 0.001	38.12 ± 4.72
0.4 g/L	Growth	0.40 ± 0.007	0.37 ± 0.06	43.14 ± 8.98	0.13 ± 0.002	0.063 ± .002	7.46 ± 1.99
	Lipid accumulation	0.60 ± 0.009	0.57 ± 0.03	130.35 ± 21.6	0.96 ± 0.088	0.194 ± 0.004	44.05 ± 11.5
	Lipid turnover	0.68 ± 0.03	0.41 ± 0.01	127.11 ± 19.5	0.99 ± 0.08	0.141 ± 0.006	44.10 ± 8.55
0.8 g/L	Growth	0.52 ± 0.008	0.57 ± 0.02	53.90 ± 7.25	0.18 ± 0.004	0.089 ± 0.04	9.27 ± 1.24
	Lipid accumulation	0.92 ± 0.01	0.79 ± 0.002	175.56 ± 21.9	1.18 ± 0.01	0.236 ± 0.002	52.49 ± 5.52
	Lipid turnover	0.96 ± 0.04	0.62 ± 0.006	174.88 ± 14.9	1.29 ± 0.02	0.181 ± 0.001	56.14 ± 2.16
1.2 g/L	Growth	0.5 ± 0.008	0.35 ± 0.06	47.5 ± 4.69	0.11 ± 0.04	0.055 ± 0.000	7.36 ± 1.25
	Lipid accumulation	0.85 ± 0.057	0.63 ± 0.008	155.74 ± 8.55	0.66 ± 0.03	0.132 ± 0.007	33.01 ± 7.27
	Lipid turnover	0.87 ± 0.061	0.48 ± 0.02	154.2 ± 5.92	0.72 ± 0.006	0.103 ± 0.002	33.30 ± 9.68
1.6 g/L	Growth	0.51 ± 0.024	0.25 ± 0.004	42.01 ± 7.91	0.08 ± 0.001	0.038 ± 0.000	6.38 ± 165
	Lipid accumulation	0.76 ± 0.084	0.36 ± 0.009	104.5 ± 13.62	0.33 ± 0.002	0.065 ± 0.000	18.39 ± 3.52
	Lipid turnover	0.61 ± 0.062	0.21 ± 0.03	78.87 ± 8.46	0.26 ± 0.06	0.037 ± 0.005	14.19 ± 2.88

All experiments were performed in triplicate. The data presented here is mean ± SD
Y _DCW growth yield (g/g glucose), P _L lipid productivity (g/L day), Y _L lipid yield (mg/g glucose), C _DHA DHA content, P _DHA biomass productivity (g/L day), Y _DHA DHA yield (g/g glucose)
^a Yield calculated on basis of glucose utilized