Fig. 2From: Characterization of a Bacillus thuringiensis chitinase that binds to cellulose and chitinEffects of pH (a, c) and temperature (b, d) on BthChi74 activity. a The activity was measured at 50 °C in various buffers at different pH values as follows: 50 mM acetate buffer (pH 3.5–6.0, diamonds), 50 mM MES-NaOH buffer (pH 5.0–7.0, circles), or 50 mM Tris–HCl buffer (pH 7.0–8.0, triangles). b The activity was measured at various temperatures (30–70 °C). c BthChi74 was incubated at 4 °C for 24 h at various pH values as follows: 50 mM acetate buffer (pH 3.5–6.0, diamonds), 50 mM MES-NaOH buffer (pH 5.0–7.0, circles), 50 mM Tris–HCl buffer (pH 7.0–9.0, triangles), or 50 mM carbonate-NaOH buffer (pH 10–11, squares). The remaining activity was measured at 50 °C after diluting the pretreated enzymes in TS buffer. The average values with error bars are represented as the enzymatic activity. Experiments were performed in triplicate. d DSF analysis results for BthChi74 are shown. The experimental procedure is detailed in the “Materials and methods” section. Experiments were performed in triplicate, and the average values are shownBack to article page