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Table 1 Physiological characterization related parameters of the PHB-producing strains TMB4443 and TMB4425 under aerobic and anaerobic conditions, respectively

From: Effect of nitrogen availability on the poly-3-d-hydroxybutyrate accumulation by engineered Saccharomyces cerevisiae

Strain

C-source 

Nitrogen supply

Aeration

Initial CDW (g/L)

Yields

PHB titer

(g/L)

PHB content

(% PHB/CDW)

YsX

(g/g C-source)

YsXylOH

(g/g C-source)

YsGlyOH

(g/g C-source)

YsAcet

(g/g C-source)

YsEtOH

(g/g C-source)

YsPHB

(mg/g C-source)

TMB4443

Glucose

No

Aerobic

3.8 ± 0.0

0.06 ± 0.00

0.00 ± 0.00

0.04 ± 0.01

0.01 ± 0.00

0.04 ± 0.01

1.0 ± 0.1

0.12 ± 0.00

1.8 ± 0.0

TMB4443

Glucose

Yes

Aerobic

0.01 ± 0.00

0.09 ± 0.01

0.00 ± 0.00

0.05 ± 0.01

0.03 ± 0.01

0.14 ± 0.07

2.12 ± 0.04

0.11 ± 0.00

2.4 ± 0.0

TMB4425

Xylose

No

Anaerobic

4.1 ± 0.2

0.06 ± 0.01

0.02 ± 0.00

0.09 ± 0.01

0.01 ± 0.00

0.01 ± 0.01

8.3 ± 0.8

0.43 ± 0.00

6.1 ± 0.1

TMB4425

Xylose

Yes

Anaerobic

0.02 ± 0.00

0.09 ± 0.00

0.04 ± 0.00

0.04 ± 0.00

0.01 ± 0.00

0.21 ± 0.03

13.8 ± 0.6

0.73 ± 0.00

16.4 ± 0.8

  1. Cell dry weight (CDW), volumetric yields and titers were calculated from a single time-point corresponding to the end of the experiment (varied from 141 to 172 h). Values reported in the table represent the mean ± SD of at least two independent biological replicates. Glucose or xylose was used as sole C-source
  2. YSX: biomass yield on C-source, YSXylOH: xylitol yield on C-source, YSGlyOH: glycerol yield on C-source, YSAcet: acetate yield on C-source, YSEtOH: ethanol yield on C-source and YSPHB: PHB yield on C-source. PHB titer: final PHB concentration. PHB content: percentage of PHB accumulated in the biomass formed