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Table 3 C/N ratios and their effects on biomass, lipid production, yield and productivity of newly isolated oleaginous yeast strains

From: Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China

Treatment

Glucose

(g/l)

Peptone

(g/l)

Yeast extract

(g/l)

Biomass

(g/l)

Lipid

(g/l)

Yield

(g/g)

Productivity

(g/l–h)

Rhodosporidium TJUWZ4

  

 20Ca

12.05

0.5

1.5

8.22 ± 0.64

1.60 ± 0.41

0.19

0.027

 20Nb

48

2

6

11.83 ± 0.22

2.86 ± 0.22

0.24

0.048

 80

48

0.5

1.5

12.4 ± 0.78

5.40 ± 1.42

0.44

0.090

 120Ca

72.3

0.50

1.5

11.54 ± 0.54

3.52 ± 0.56

0.31

0.059

 120Nb

48

0.33

0.99

13.32 ± 1.19

3.86 ± 0.05

0.29

0.064

Cryptococcus TJUWZA11

  

 20Ca

14

2

0

8.92 ± 0.73

0.91 ± 0.25

0.10

0.015

 20Nb

56

8

0

8.2 ± 0.43

0.75 ± 0.02

0.09

0.013

 80

56

2

0

9.64 ± 0.41

1.19 ± 0.11

0.12

0.020

 120Ca

84

2

0

9.38 ± 0.81

1.02 ± 0.18

0.11

0.017

 120Nb

56

1.33

0

10.16 ± 0.78

1.54 ± 0.43

0.15

0.026

  1. C/N calculation formula: \({{{\text{C}}/{\text{N}} = \left[ {\text{Glu}} \right] \times 0. 4 { }\left( {{\text{g}} - {\text{C}}/{\text{g}} - {\text{Glu}}} \right)} \mathord{\left/ {\vphantom {{{\text{C}}/{\text{N}} = \left[ {\text{Glu}} \right] \times 0. 4 { }\left( {{\text{g}} - {\text{C}}/{\text{g}} - {\text{Glu}}} \right)} {\left\{ {\left( {\left[ {\text{Pep}} \right]{\text{ x }}0. 1 4 { }\left( {{\text{g}} - {\text{N}}/{\text{g}} - {\text{Pep}}} \right)} \right) + \left( {\left[ {\text{YE}} \right]{\text{ x }}0. 1 1 4 { }\left( {{\text{g wt}}.{\text{ N in YE}}} \right)} \right)} \right\}}}} \right. \kern-0pt} {\left\{ {\left( {\left[ {\text{Pep}} \right]{\text{ x }}0. 1 4 { }\left( {{\text{g}} - {\text{N}}/{\text{g}} - {\text{Pep}}} \right)} \right) + \left( {\left[ {\text{YE}} \right]{\text{ x }}0. 1 1 4 { }\left( {{\text{g wt}}.{\text{ N in YE}}} \right)} \right)} \right\}}}\)
  2. aC means that altering C/N ratio by changing carbon source contents at a constant concentration of nitrogen source
  3. bN means that altering C/N ratio by changing nitrogen source contents at a constant concentration of carbon source