Table 1 Primer sequences and probes designed in the study
From: Development of a SYBR Green I real-time PCR for the detection of the orf virus
Primer | Sequence (5′ → 3′) | Genomic position | Product length (bp) |
|---|---|---|---|
F1 R1 | CGGAATTCAGTCCGCGAAGAAGTTTTTG CCCTCGAGGCGAGTCCGAGAAGAATACG | 125 622 | 514 |
F2 R2 | GGGCTCTACTCCACCAACAA CGAGTCCGAGAAGAATACGC | 442 621 | 180 |