Table 4 Phylogenetic affiliations and number of bacterial clones obtained from retained and suspended fractions in the MFC operated for 30 days at pH 7.0
OTUa | No. of clonesb | Phylogenetic group | The closest isolated strain (accession no., similarity) | The closest environmental clone (accession no., similarity) | Isolated source of environmental clones | |
|---|---|---|---|---|---|---|
Retained | Suspended | |||||
1 | 6 | Â | Proteobacteria; Deltaproteobacteria | Geobacter sulfurreducens (NR_075009, 98.6%) | Uncultured bacterium (GQ152932, 100%) | Mixed consortium in MFC |
2 | 4 | Â | Proteobacteria; Gammaproteobacteria | Citrobacter freundii (AB548830, 100%) | Uncultured organism (HQ745055, 99.9%) | Gastrointestinal specimens |
3 | 3 | 1 | Proteobacteria; Gammaproteobacteria | Raoultella ornithinolytica (CP004142, 100%) | Uncultured bacterium (EF515418, 100%) | Upflow MFC anode |
4 | 4 | 1 | Proteobacteria; Gammaproteobacteria | Pseudomonas fragi (AB685617, 99.8%) | Uncultured bacterium (EU469649, 99.8%) | Mammalian gut microbes |
5 | Â | 5 | Proteobacteria; Gammaproteobacteria | Aeromonas dhakensis (KF938660, 100%) | Uncultured bacterium (JX271966, 100%) | Activated sludge from large-scale |
6 | Â | 3 | Proteobacteria; Gammaproteobacteria | Aeromonas sharmana (NR_043470, 96.9%) | Uncultured Aeromonas sp. (EF679186, 98.6%) | Sewage plant Anaerobic sludge from MFC |
7 | 1 | Â | Proteobacteria; Betaproteobacteria | Advenella kashmirensis (KF956701, 95.1%) | Uncultured bacterium (FN563161, 99.7%) | Mesophilic biogas digester |
8 | 1 | 1 | Proteobacteria; Betaproteobacteria | Azospira oryzae (DQ863512, 94.1%) | Uncultured Azospira sp. (JF736645, 96.5%) | Biofilm on electrode in MFC |
9 | 12 | 5 | Bacteroidetes; Cytophagia | Pontibacter korlensis (GQ503321, 82.7%) | Bacteriodetes bacterium (JX828412, 95.9%) | Iron-reducing enrichment culture |
10 | 3 | Â | Bacteroidetes; Bacteroidia | Bacteroides nordii (NR_112939, 92.9%) | Uncultured bacterium (KC179060, 99.7%) | Activated sludge |
11 | Â | 12 | Bacteroidetes; Bacteroidia | Dysgonomonas oryzarvi (NR_113063, 99.7%) | Uncultured Dysgonomonas sp. (JX535216, 100%) | Activated sludge from sewage |
12 | Â | 1 | Bacteroidetes; Bacteroidia | Dysgonomonas capnocytophagoides (NR_113133, 93.2%) | Uncultured bacterium (HQ728219, 96.1%) | Glucose-fed MFC |
13 | 3 | Â | Firmicutes; Negativicutes | Anaeroarcus burkinensis (NR_025298, 97.7%) | Uncultured bacterium (AB329655, 99.9%) | UASB granule |
14 | 2 | Â | Firmicutes; Negativicutes | Acidaminococcus fermentans (NR_074928, 93.8%) | Uncultured bacterium (JN680060, 99.5%) | Anode biofilm at psychrotolerants in MFC |
15 | Â | 1 | Firmicutes; Clostridia | Pseudoflavonifractor capillosus (NR_025670, 89.8%) | Uncultured Clostridiales bacterium (FJ393118, 91.0%) | Glucose-fed MFC anode |
Sum | 39 | 30 | Â | Â | Â | Â |