Table 1 Identification of the isolates on the basis of conventional and molecular analysis
Isolate ID | Growth characteristics on different differential and selective media | Microscopic characteristics | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Media | Appearance | Form | Elevation | Margin | Consistency | Gram-staining | Size | Shape | Arrangement | |
28N, 26N, CR1, CR2, CR4, CR6, NCX9, MCX14, C6, C49, C79, C84, E8, E23, E29, E34, E36, E37, E40, E41, E42, E56, E58, G2, G3, G4 | MAC | DP | C | F | E | D | Gram-negative | S | SR | Single |
EMB | BB, GMS | |||||||||
XLD | Y | |||||||||
MCX1, MCX2, MCX3, MCX4, MCX5, MCX6, NCX4 | MAC | P | C | R | E | G | M | Rd | ||
EMB | B, DC | |||||||||
XLD | Y | |||||||||
NCX6, MCX10, C1, C67, E33 | MAC | LP | C | F | E | D | M | Rd | ||
EMB | B, DC | C | F | E | G | |||||
XLD | Y | C | F | E | D | |||||
18, 20, 36, 44, 45, 49, 54, 58, 60,68, 74, 77, 81, 84, 94 | MAC | Colorless | C | F | E | D | M | Rd | ||
EMB | Color less | C | F | E | G | |||||
XLD | Red, BC | C | F | E | D | |||||
Isolate ID | Biochemical characteristics | Presumptive organism | ARDRA genotype | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sugar utilization tests | IMViC tests | Oxidase Test | KIA Test | Motility | |||||||||||
Gl | Su | La | Indole | MR | VP | Citrate | Slant | Butt | Gas | H2S | |||||
28N, 26N, CR1, CR2, CR4, CR6, NCX9, MCX14, C6, C49, C79, C84, E8, E23, E29, E34, E36, E37, E40, E41, E42, E56, E58, G2, G3, G4 | + | ± | + | + | + | − | − | − | Y | Y | + | − | + | Escherichia spp. | I |
MCX1, MCX2, MCX3, MCX4, MCX5, MCX6, NCX4 | + | − | + | − | − | + | + | − | Y | Y | + | − | − | Enterobacter spp. | II |
NCX6, MCX10, C1, C67, E33 | + | − | + | − | − | + | + | − | Y | Y | + | − | − | Klebsiella spp. | III |
18, 20, 36, 44, 45, 49, 54, 58, 60,68, 74, 77, 81, 84, 94 | + | − | − | − | + | − | + | − | Red | Y | − | + | − | Salmonella spp. | IV |