Purification and characterization of a novel ginsenoside Rc-hydrolyzing β-glucosidase from Armillaria mellea mycelia

AMB Express

Table 3 ¹³C-NMR chemical shifts of hydrolysis products of G-Rc by BG-1

Carbon no	G-Rd	C-Mc1	G-F2	C-Mc	C-K
Aglycone moiety
1	39.0	39.6	38.9	39.9	39.8
2	26.6	27.2	26.5	26.6	26.6
3	88.8	89.2	88.6	78.5	78.7
4	39.5	40.1	39.5	39.8	39.9
5	56.2	56.8	56.1	56.6	56.8
6	18.3	18.2	18.2	18.3	19.2
7	35.0	35.5	34.9	35.6	35.6
8	39.9	40.4	39.8	40.0	40.0
9	50.0	50.6	50.0	50.7	50.8
10	36.7	37.3	36.7	37.8	37.8
11	30.6	30.6	30.6	30.6	31.3
12	70.1	70.6	70.1	70.6	70.6
13	49.3	49.8	49.5	49.9	50.0
14	51.4	52.0	51.2	51.8	52.0
15	30.7	30.4	29.8	31.1	31.4
16	26.5	27.0	26.4	26.2	26.3
17	51.5	51.8	51.5	52.1	51.9
18	16.1	16.7	16.1	16.7	16.5
19	15.8	16.4	15.7	16.5	16.8
20	83.2	82.8	83.1	83.8	83.8
21	22.3	22.3	22.3	22.8	22.8
22	35.9	36.5	35.8	36.6	36.7
23	23.1	23.6	23.1	23.6	23.7
24	125.8	126.4	125.7	125.3	126.5
25	130.8	131.3	130.8	131.4	131.4
26	25.7	26.2	25.6	26.2	26.2
27	17.6	17.2	17.6	17.8	17.9
28	27.9	28.5	27.9	27.2	28.8
29	16.5	16.7	16.6	16.8	16.7
30	17.2	17.8	17.1	17.8	17.6
3-Glucopyranosyl (inner)
1′	105.0	105.4	106.7
2′	83.2	76.2	75.6
3′	78.1	79.3	77.8
4′	71.5	72.3	71.7
5′	78.1	79.1	78.2
6′	62.6	63.0	62.8
3-Glucopyranosyl (outer)
1″	105.9
2″	77.0
3″	79.1
4″	71.5
5″	78.1
6″	62.7
20-Glucopyranosyl
1′	98.1	98.5	98.1	98.5	98.7
2′	75.0	75.2	75.0	75.4	75.6
3′	77.8	78.6	79.0	79.7	77.8
4′	71.4	72.1	71.3	72.6	72.1
5′	78.0	76.9	78.5	77.0	78.5
6′	62.5	68.8	62.5	68.9	63.4
20-Arabinofuranosyl
1″		110.5		110.5
2″		83.7		83.7
3″		78.7		78.6
4″		86.5		86.5
5″		63.0		83.1