Fig. 3From: Development of a novel strategy for fungal transformation based on a mutant locus conferring carboxin-resistance in Magnaporthe oryzae Cytoplasmic eGFP expression at different developmental stages in Mosdi1 ReGFP mutants. All the samples for microscopic examination were treated as described in the main text and observed by a manual inverted fluorescent microscope (Ti-S, Nikon, Tokyo, Japan). Images from all independent transformants showed equivalent fluorescent signal in hyphae, conidia, germ tube, appressorium and invasive hyphae after integration of pMoC-eGFP into the Mosdi1 locus. Scale bar 30 μmBack to article page