pH | Relative densities of hydrolysis spots produced by lipase from different sources | ||||||||
---|---|---|---|---|---|---|---|---|---|
C. antarctica | C. rugosa | M. javanicus | Porcine pancreas | P. cepacia | P. fluorescens | R. arrhizus | R. niveus | R. oryzae | |
Buffer | 1.00 ± 0.30a | 1.00 ± 0.15a | 1.00 ± 0.32a | 1.00 ± 0.11a | 1.00 ± 0.15a | 1.00 ± 0.27a | 1.00 ± 0.18a | 1.00 ± 0.20a | 1.00 ± 0.21a |
1 | 0.70 ± 0.87a | 0.82 ± 0.24a | 1.05 ± 0.30a | 0.84 ± 0.77a | 0.72 ± 0.49a | 1.26 ± 0.79a | 5.77 ± 0.42abc | 3.90 ± 0.21ab | 1.19 ± 0.39a |
2 | 1.35 ± 0.41a | 0.89 ± 0.26a | 1.03 ± 0.20a | 0.55 ± 0.50a | 0.88 ± 1.28a | 1.09 ± 0.45a | 5.53 ± 0.57abc | 2.03 ± 0.32a | 1.15 ± 0.14a |
3 | 25.98 ± 0.30d | 0.80 ± 0.11a | 1.10 ± 0.55a | 1.04 ± 0.47a | 2.53 ± 0.15a | 1.28 ± 0.91a | 6.03 ± 0.53abc | 6.41 ± 0.54abc | 1.19 ± 0.29a |
4 | 14.27 ± 0.20bc | 4.24 ± 0.26b | 0.83 ± 0.28a | 0.91 ± 0.65a | 15.51 ± 0.40abcd | 2.29 ± 0.30ab | 27.65 ± 0.49bcd | 8.24 ± 0.23bcd | 1.20 ± 0.20a |
5 | 20.93 ± 0.25 cd | 6.11 ± 0.09c | 3.56 ± 0.42ab | 0.81 ± 0.53a | 19.05 ± 0.10bcd | 4.36 ± 0.34ab | 26.77 ± 0.59bcd | 11.51 ± 0.36cde | 1.20 ± 0.16a |
6 | 30.66 ± 0.30d | 7.26 ± 0.05c | 3.78 ± 0.39ab | 5.30 ± 0.24bc | 17.47 ± 0.25abcd | 4.48 ± 0.28ab | 29.37 ± 0.04 cd | 13.35 ± 0.13de | 5.19 ± 0.60b |
7 | 51.64 ± 0.01e | 6.77 ± 0.09c | 8.35 ± 0.41b | 8.91 ± 0.36c | 20.85 ± 0.20 cd | 7.45 ± 0.48abc | 23.60 ± 0.77abcd | 14.79 ± 0.26e | 3.77 ± 0.40ab |
8 | 6.40 ± 0.39ab | 0.83 ± 0.33a | 6.53 ± 0.69b | 8.58 ± 0.39c | 11.45 ± 0.31abc | 17.59 ± 0.30c | 35.18 ± 0.22d | 17.34 ± 0.03e | 5.20 ± 0.15b |
9 | 1.20 ± 0.23a | 0.71 ± 0.22a | 0.91 ± 0.29a | 3.86 ± 0.22ab | 10.15 ± 0.26abc | 17.26 ± 0.25c | 24.28 ± 0.20abcd | 1.38 ± 0.42a | 6.69 ± 0.08b |
10 | 0.99 ± 0.27a | 0.80 ± 0.13a | 0.88 ± 0.55a | 1.32 ± 0.41a | 8.27 ± 0.02abc | 18.26 ± 0.41c | 3.15 ± 0.31ab | 1.27 ± 0.25a | 1.17 ± 0.35a |
11 | 0.86 ± 0.38a | 0.66 ± 0.30a | 1.10 ± 0.62a | 0.49 ± 0.53a | 30.88 ± 0.57d | 13.10 ± 0.57bc | 3.00 ± 0.11ab | 1.56 ± 0.47a | 1.08 ± 0.24a |