Table 1 Purification of NecC from T. discophora
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Mycelium extract | 363.9 | 40.7 | 0.11 | 100 | 1.0 |
Ammonium sulfate precipitation | 49.4 | 43.1 | 0.87 | 106 | 7.8 |
DEAE chromatography | 14.9 | 10.0 | 0.67 | 25 | 6.0 |