Glycolipids produced by Rouxiella sp. DSM 100043 and isolation of the biosurfactants via foam-fractionation

AMB Express

Table 1 NMR data of fatty acids moieties

	C Shift [ppm]	H Shift [ppm]	Multiplicity	Coupling [Hz]
3′ OH C12:1 FA
O=C¹–OH	175.95
–C²H₂–	42.39	2.46, 2.24	dd, dd	15.4, 8.25, 4.6
–C³H–OH	69.6	4.00	m
–C⁴H₂–	35.8	2.26	m
–C⁵H=	125.7	5.43	m
–C⁶H=	133.5	5.50	m
–C⁷H₂–	28.3	2.05	q	7.1
–C⁸H₂–	30.7	1.37	m
–C⁹H₂–	30.1	1.34	m
–C¹⁰H₂–	32.9	1.31	m
–C¹¹H₂–	23.7	1.33	m
–C¹²H₃	14.4	0.90	t	6.8
C14/C14:1 FA
O=C¹–OH	177.8
–C²H₂–	35.1	2.27	t	7.4
–C³H₂–	26.1	1.59	m
–C⁴H₂–	30.26	1.33
–C⁵H₂–	30.6	1.33
–C⁶H₂–	30.3	1.33
–C⁷H₂–	31.1	1.33	m
–C⁸H₂–	28.17	2.03	dd	12.3, 6.4
–C⁹H₂–/–C⁹H=	130.9	5.34
–C¹⁰H₂–/–C¹⁰H=	130.9	5.34
–C¹¹H₂–	28.17	2.03	dd	12.3, 6.4
–C¹²H₂–	33.1	1.29	m
–C¹³H₂–	23.8	1.32	m
–C¹⁴H₃	14.6	0.89	t	6.6

Chemical shifts of carbon and hydrogen nuclei, multiplicity of the peak observed and its coupling constant of 3′ hydroxy lauroleic acid from fractions 60–61 (3′OH C12:1 FA) and potential myristic/myristoleic acid from farctions 67–69 (C14/C14:1 FA)
FA fatty acid, d doublet, t triplet, q quartett, m multiplet