Table 2 Experimental variables at different levels used for production of Melanin
From: Improved production of melanin from Aspergillus fumigatus AFGRD105 by optimization of media factors
Factors | Variable | Units | Experimental values | |
|---|---|---|---|---|
Low (−1) | High (+1) | |||
1 | pH | 5 | 9 | |
2 | Temperature | °C | 40 | 50 |
3 | Inoculum volume | CFU/Ml | 0.5 | 1.5 |
4 | Incubation time | Days | 3 | 7 |
5 | Substrate | g | 3 | 7 |
6 | Moisture content | % | 10 | 25 |
7 | Sodium dihydrogen phosphate | g | 10 | 14 |
8 | Potassium dihydrogen phosphate | g | 1 | 3 |
9 | Magnesium sulphate | g | 0.1 | 0.5 |
10 | Calcium chloride | g | 0.5 | 1.0 |
11 | Ferrous sulphate | g | 0.1 | 1 |