Fig. 5
From: Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes
a Colony PCR after affinity selection of M13-toxins phage particles. A mixture of M13-Cry1Ac and M13-Cyt1Aa phages (1:1) was applied to immobilized cadherin fragment CR7-12 (1), anti-Cry1Ac antibody (2) and anti-Cyt1Aa antibody (3). E. coli XL1-Blue MRF’ cells were infected with the output phages and ten random colonies were analyzed by PCR to amplify cry1Ac or cyt1Aa. b RT-PCR products after selection of ternary complex prepared with a mixture of Cry1Ac and Cyt1Aa mRNA (1:1) against cadherin fragment CR7-12, anti-Cry1Ac antibody, anti-Cyt1Aa antibody or Cry11Aa toxin. Lane 1 RT-PCR preformed with specific primers for Cry1Ac toxin; lane 2 RT-PCR performed with specific primers for Cyt1Aa toxin. MWM Mass weight marker GeneRuler 1 kb DNA Ladder