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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization

Fig. 3

a A chromatographic runs of AviPure purification with 5 mL Ni–NTA column, (1) load, (2) wash, (3) elution fraction. b Coomassie Blue stained 15 % SDS-PAGE gel from different sample fractions, Lane M represent pre-stained protein marker, Lane 1 induced pellets from culture, Lane 2 supernatant after sonication and centrifugation, Lane 3 flow through, Lane 4 Wash, Lane 5 eluted sample before dialysis, Lane 6 eluted sample after dialysis

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