Fig. 2From: Engineering of Nitrosomonas europaea to express Vitreoscilla hemoglobin enhances oxygen uptake and conversion of ammonia to nitritepSK2 is stably maintained in N. europaea[pSK2]. Lane 1 pSK2 plasmid prep from N. europaea [pSK2]; lane 2 positive control PCR of vgb amplicon (from pUC8:16 from E. coli); lane 3 negative control PCR of vgb (plasmid prep from untransformed N. europaea); lane 4 PCR amplicon of vgb from N. europaea [pSK2]; lane 5 2-log ladder (New England Biolabs; position of 500 bp fragment is noted). The amplicons in lanes 2 and 4 are of the expected size (about 450 bp).Back to article page