Table 1 Bacterial strains and plasmids used in this study
From: Engineering alternative isobutanol production platforms
Strains | Genotype | References |
|---|---|---|
Escherichia coli DH10B | F−, mcrA, Δ(mrr-hsdRMS-mcrBC), f80ΔlacZDM15 ΔlacX74, deoR, recA1, endA1, araD139, Δ(ara,leu)7697, galU, galK, rpsL, nupG, λ − | Invitrogen |
Escherichia coli W | Type strain. Waksman’s strain | ATCC9637 |
Shimwellia blattae | Type strain | CIP 104942, DSMZ 4481 |
Plasmids | ||
pUC57 | ApR, pUC-derived cloning vector for E. coli bearing Plac. rep (pMB1) | Genscript |
pIZ1016 | GmR, broad-host range expression vector bearing lacIq and Ptac. rep (pBBR1MCS) | Martínez-Perez et al. (2004) |
pSEVA424 | SmR/SpR, broad-host range expression vector bearing lacIq and Ptrc.rep (RK2 replicative origin. oriV-trfA) | Silva-Rocha et al. (2013) |
pUC57-IbPSO | ApR, IbPSO into pUC57 | This work |
pIZIbPSO | GmR, IbPSO into pIZ1016 | This work |
p424IbPSO | SmR/SpR, IbPSO into pSEVA424 | This work |