Figure 5
From: Functional screening system for yeast-secreted peptides acting on G-protein coupled receptors
Model screening of Ex4-secreting yeast using the direct activity detection system. a Schematic illustration for model screening of Ex4-yeast was indicated. b We prepared 16 wells including 9:1 yeast mixture (10 cells in total per well) in the ratio of Ctrl-yeast to Ex4-yeast and conducted GLP1R activation assay according to the workflow indicated above. Only Ctrl-yeast or Ex4-yeast was tested as a control. c The existence of Ex4-yeast in the positive and negative wells was investigated by colony-direct PCR after the colony formation of yeasts from both wells (numbering from 1 to 24 means the number of colony picked up). The representative electrophoresis data are shown for colonies from a positive and a negative well, respectively. M molecular size marker (New England Biolabs, Ipswich, MA, USA).