Figure 4
From: Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function
IL-6 (A) and PGE2 (B) production by affibody protein-introduced MH7A cells. MH7A cells were transfected with affibody proteins. After 24 h of culture, the cells were stimulated with TNF-α (100 ng/mL) and cultured for an additional 24-48 h. IL-6 and PGE2 levels were analyzed, and percent inhibition of IL-6 or PGE2 production was determined. Percent inhibition was calculated as follows: ([(IL-6 or PGE2 levels in affibody protein-introduced MH7A cells) - (IL-6 or PGE2 levels in MH7A cells introduced with standard peptides)]/([IL-6 or PGE2 levels in MH7A cells introduced with standard peptides]) × 100. Data represent the means × SD of three independent experiments. *p <0.05.