Figure 2
From: Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function
IL-6 (A) and PGE2 (B) production by MH7A cells with plasmid-expressed affibodies. MH7A cells were transfected with plasmids encoding affibodies. After 24 h of culture, the cells were stimulated with TNF-α (100 ng/mL) and cultured for an additional 24-48 h. Data represent the percent inhibition of IL-6 or PGE2 production by MH7A cells. Percent inhibition was calculated as follows: ([(IL-6 or PGE2 levels in MH7A cells transfected with affibody DNA) - (IL-6 or PGE2 levels in MH7A cells transfected with pcDNA3.1)]/[IL-6 or PGE2 levels in MH7A cells transfected with pcDNA3.1]) × 100. Data represent the means × SD of three independent experiments. *p <0.05.