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Table 2 Apparent kinetic parameters for FBP and MgCl 2 at pH 8.0 a

From: Diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three Gram-negative bacteria

 

kcat(s-1)

vmin(s-1)b

S0.5(mM)

n H c

FBP

    

FNLDH

45 (1)

35 (0.6)

4.2 (0.2)

1.7 (0.05)

PALDH

150 (30)

100 (2)

12 (3)

1.3 (0.07)

ECLDH

25 (1)

180 (2)

1.3 (0.01)

1.5 (0.2)

Mg2+

    

FNLDH

22 (0.7)

37 (0.2)

1.5 (0.1)

1.3 (0.09)

PALDH

140 (2)

100 (1)

0.16 (0.008)

1.1 (0.08)

ECLDH

N.D.d

N.D.

N.D.

 
  1. aParameters were determined as described in the `Materials and methods' section, and standard deviations are shown in parentheses. bvmin is reaction velocity in the absence of FBP or Mg2+. cHill coefficients (nH) are shown only for the cases that exhibited significant cooperativity. dN.D., not determined.
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