| kcat(s-1) | S0.5(mM) | kcat/S0.5(s-1 mM-1) | Ki(mM)b | n H c |
---|---|---|---|---|---|
Glyoxylate | Â | Â | Â | Â | Â |
FNLDH | 11 (0.1) | 18 (0.4) | 0.70 (0.007) | Â | Â |
PALDH | 880 (60) | 6.8 (0.6) | 130 (5) | 44 (8) | Â |
ECLDH | 100 (5) | 20 (2) | 5.3 (0.2) | Â | 1.5 (0.09) |
Pyruvate | Â | Â | Â | Â | Â |
FNLDH | 80 (0.4) | 0.34 (0.007) | 230 (4) | Â | Â |
PALDH | 400 (10) | 0.10 (0.009) | 4,000 (300) | 1.8 (0.1) | Â |
ECLDH | 410 (8) | 2.6 (0.09) | 160 (2) | Â | 1.4 (0.05) |
2-Ketobutyrate | Â | Â | Â | Â | Â |
FNLDH | 140 (0.7) | 0.31 (0.006) | 440 (7) | Â | Â |
PALDH | 100 (0.6) | 0.64 (0.01) | 150 (3) | Â | Â |
ECLDH | 31 (2) | 30 (4) | 1.0 (0.05) | Â | 1.4 (0.08) |
2-Ketovalerate | Â | Â | Â | Â | Â |
FNLDH | 140 (0.5) | 0.68 (0.01) | 200 (3) | Â | Â |
PALDH | 69 (0.5) | 4.0 (0.07) | 18 (3) | Â | Â |
ECLDH | N.D.d | N.D. | <0.01 | Â | Â |
2-Ketoisovalerate | Â | Â | Â | Â | Â |
FNLDH | 120 (0.7) | 3.5 (0.07) | 35 (0.5) | Â | Â |
PALDH | N.D. | N.D. | <0.4 | Â | Â |
ECLDH | N.D. | N.D. | <0.01 | Â | Â |
2-Ketocaproate | Â | Â | Â | Â | Â |
FNLDH | 230 (1) | 2.6 (0.06) | 87 (1) | Â | Â |
PALDH | 160 (1) | 5.8 (0.1) | 27 (0.4) | Â | Â |
ECLDH | N.D. | N.D. | <0.01 | Â | Â |
2-Ketoisocaproate | Â | Â | Â | Â | Â |
FNLDH | 200 (2) | 3.8 (0.1) | 53 (1) | Â | Â |
PALDH | 39 (0.3) | 12 (0.2) | 3.2 (0.03) | Â | Â |
ECLDH | N.D. | N.D. | <0.01 | Â | Â |
Oxaloacetate | Â | Â | Â | Â | Â |
FNLDH | 80 (1) | 2.5 (0.09) | 31 (0.7) | 140 (20) | Â |
PALDH | 410 (4) | 1.7 (0.06) | 230 (6) | Â | Â |
ECLDH | 1,100 (200) | 54 (10) | 21 (0.6) | 5.0 (1) | Â |
Hydroxypyruvate | Â | Â | Â | Â | Â |
FNLDH | 160 (2) | 18 (0.4) | 8.7 (0.07) | Â | Â |
PALDH | 420 (10) | 3.0 (0.2) | 140 (3) | 19 (1) | Â |
ECLDH | 50 (0.5) | 12 (0.3) | 4.2 (0.06) | Â | Â |
Phenylpyruvate | Â | Â | Â | Â | Â |
FNLDH | 120 (7) | 11 (0.9) | 11 (0.3) | 23 (3) | Â |
PALDH | 18 (0.2) | 7.1 (0.2) | 2.6 (0.04) | Â | Â |
ECLDH | N.D. | N.D. | <0.01 | Â | Â |
Hydroxyphenylpyruvate | Â | Â | Â | Â | Â |
FNLDH | N.D. | N.D. | <0.02 | Â | Â |
PALDH | N.D. | N.D. | <0.4 | Â | Â |
ECLDH | N.D. | N.D. | <0.01 | Â | Â |