Figure 3

SDS-PAGE and western blot analysis of G. graminis LOX expression A. nidulans . Samples T1-T3 are transformants of A. nidulans carrying the G. graminis lox gene, while M1-M3 are transformants of A. nidulans carrying the mutated G. graminis lox gene. As positive controls 10 ng, 100 ng, and 1000 ng of the G. graminis LOX were applied. The wild type (WT) sample is used as a negative control. a) SDS-PAGE of the proteins in the culture broth of A. nidulans; b) SDS-PAGE of intracellular proteins of A. nidulans; c) Western blot of the proteins in the culture broth reacting to the antibodies of the G. graminis LOX; d) Western blot of intracellular proteins reacting to the antibodies of G. graminis LOX.