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AMB Express
Figure 2 | AMB Express

Figure 2

From: Cloning, expression and characterization of a versatile Baeyer-Villiger monooxygenase from Dietzia sp. D5

Figure 2

Expression and purification of BVMO4. a) Soluble crude extracts of different E. coli strains expressing BVMO4. Lane 1 and 6, protein marker (AllBlue, BioRad); lane 2, E. coli BL21(DE3); lane 3, E. coli Rosetta(DE3); lane 4, E. coli ArcticExpress(DE3)-RP; and lane 5, E. coli BL21-CodonPlus(DE3)-RP. b) Purified BVMO4. Lane 1 and 5, protein marker (AllBlue, BioRad); lane 2, cell debris; lane 3, soluble cell extract; lane 4, purified protein after His-tag affinity chromatography.

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