Figure 3From: Properties of the newly isolated extracellular thermo-alkali-stable laccase from thermophilic actinomycetes, Thermobifida fusca and its application in dye intermediates oxidation Purification of laccase by column chromatography. (a) Q-Sepharose FF chromatography. (●) laccase activity; (○) Protein (mg/ml); (−) NaCl gradient. Column: 1.13 × 5 cm; flow rate: 180 ml/h. (b) Sephacryl™ S-200 chromatography. (●) laccase activity; (○) Protein (mg/ml); (−) NaCl gradient. Column: 1.6 × 90 cm; flow rate: 30 ml/h.Back to article page