Bacillus subtilis natto: a non-toxic source of poly-γ-glutamic acid that could be used as a cryoprotectant for probiotic bacteria

AMB Express

Table 1 PCR primers used and virulence factors (adapted from Matarante et al. 2004 )

Target gene	Primer name	Primer sequence (5′-3′)	Amplicon size (bp)	Reference
hbl – D/A	hblD-f	GGAGCGGTCGTTATTGTTGT	623	(Matarante et al. 2004)
hbl – D/A	hblA-r	GCCGTATCTCCATTGTTCGT	623	(Matarante et al. 2004)
nheB	nheB 1500 S	CTATCAGCACTTATGGCAG	769	(Matarante et al. 2004)
nheB	nhe B 2269 A	ACTCCTAGCGGTGTTCC	769	(Matarante et al. 2004)
bceT	ETF	TTACATTACCAGGACGTGCTT	428	(Matarante et al. 2004)
bceT	ETR	TGTTTGTGATTGTAATTCAGG	428	(Matarante et al. 2004)
entFM	EntA	ATGAAAAAAGTAATTTGCAGG	1269	(Matarante et al. 2004)
entFM	EntB	TTAGTATGCTTTTGTGTAACC	1269	(Matarante et al. 2004)
sph	Ph1	CGTGCCGATTTAATTGGGGC	558	(Matarante et al. 2004)
sph	Ph2	CAATGTTTTAAACATGGATGCG	558	(Matarante et al. 2004)
piplc	PC105	CGCTATCAATGGACCATGG	569	(Matarante et al. 2004)
piplc	PC106	GGACTATTCCATGCTGTACC	569	(Matarante et al. 2004)
pfkA*	pfkA-F	CCATCAGCTAAACCAGCC	370	This study
pfkA*	pfkA-R	CGCGGTGGTACGAAATTA	370	This study