Table 1 Selected examples of the different reactions used by suppliers to test lipase activity
Commercial lipase preparation | Supplier | Physical state | Lipase activity | Test reaction | Definition of Unit | Reference |
|---|---|---|---|---|---|---|
CRL (Lipase AY 30) | Amano | powder | > 30000 U/g | Not specified hydrolysis | Amount of enzyme that releases 1 μmol of fatty acid per minute at pH 7, not specified temperature | Technical sheet |
CRL (VII ) | Sigma Chemicals Co. | powder | 900000 U/g | Hydrolysis of olive oil | Amount of powder which produces 1 μmol of oleic acid per hour at pH=7.2 and 37°C. | Arroyo (1995) |
Lipozyme RM IM (RML) | Novozymes | immobilized | > 150 U/g | Interesterification of soybean oil, no co-solvents | 0.01 w/w% converted tristearin/min-g enzyme. | Technical sheet |
Novozym 435 (CALB) | Novozymes | immobilized | ~ 10000 U/g | Synthesis of propyl laureate | n.a. | Technical sheet |