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Table 3 Specific activities and ranking of lipases in P(3HB- co -92 mol% 4HB), p NPL and olive oil hydrolysis assay

From: Densitometry based microassay for the determination of lipase depolymerizing activity on polyhydroxyalkanoate

Sources of lipases

P(3HB- co-92 mol% 4HB)

p NPL

 

Olive oil

 
 

Specific activity (Relative density/ min/ mg)

Rank*

Specific activity (μmol 4-nitrophenol/ min/ mg)

Rank†

Specific activity (μmol fatty acid/ min/ mg)

Rank #

C. viscosum

4369.47

1

9800

1

365.00

1

C. antarctica

596.29

2

44

4

0.60

11

M. miehei

249.67

3

8.1

6

49.35

2

P. cepacia

58.76

4

80

2

15.28

3

R. oryzae

52.8

5

0.1

10

6.19

6

R. arrhizus

45.52

6

6.2

7

10.69

4

Porcine pancreas

32.19

7

0.25

8

0.90

10

P. fluorescens

23.97

8

37

5

5.64

7

M. javanicus

21.05

9

0.15

9

2.83

8

C. rugosa

15.21

10

68

3

8.72

5

R. niveus

1.06

11

0.03

11

1.53

9

  1. * Rank according to specific activity by using P(3HB-co-4HB) as substrate, †Rank according to specific activity by using p-NPL as substrate, #Rank according to specific activity by using olive oil as substrate.