Figure 2

Electrophoretic patterns of cell crude extracts during growth of auto-induced pCM13(SELP-1020-A)-BL21(DE3) cultures in A) LB+lac at 37°C, B) TB+lac at 37°C and C) TB+lac at 30°C. To facilitate direct comparison of protein expression, all samples were normalised for the same cell density before loading on gel. The sampling time is given above each lane whereas the OD600 at that time point is indicated below. Target recombinant protein is indicated by arrows and the molecular weight marker is in the left of each gel. The abnormal gel mobility of the recombinant protein was previously observed by other authors (Teng et al. 2009; Lyons et al. 2007; McPherson et al. 1992).