AMB Express

Table 2 Linearity of qPCR calibration curves

From: Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide

Species	R ²	E
S. oralis	0.999	0.915
S. gordonii	0.998	0.920
V. parvula	0.999	0.950
F. nucleatum	0.999	0.950
P. intermedia	0.999	0.950

R² indicates the regression coefficient. PCR efficiency (E) is obtained from E= 10^-1/s – 1, where s is the slope of the regression line between Cp values and log cells (Knutsson et al., 2002).
Cp values versus log (number of cells) of five oral bacterial species.

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