Table 1 Purification of MnP isoenzymes from Bjerkandera sp. (A), Phanerochaete chrysosporium (B), Physisporinus rivulosus (C), Phlebia radiata (D) and Phlebia sp. Nf b19 (E)
Activity [U] | Protein [mg] | Specific activity [U/mg] | Activity protein absorbance ratio [UL-1 absorbance at 280nm-1] | Yield [%] | Purification fold | Size of MnP [kDa] | |
|---|---|---|---|---|---|---|---|
A | |||||||
Culture fluid | 38.0 | 2.9 | 13.3 | 100 | 1.0 | ||
Ultrafiltration | 19.8 | 2.6 | 7.6 | 52.2 | 0.6 | ||
Mono Q BSMnP1 | 15.3 | 0.5 | 32.3 | 18 | 40.2 | 2.4 | 45 |
B | |||||||
Culture fluid | 38.7 | 1.447 | 26.7 | 100 | 1.0 | ||
Ultrafiltration | 6.9 | 0.403 | 17.0 | 17.7 | 0.6 | ||
Mono Q PCMnP1 | 1.1 | 0.005 | 200.5 | 13 | 2.8 | 7.5 | 45 |
Mono Q PCMnP2 | 0.4 | 0.013 | 33.0 | 8 | 1.1 | 1.2 | 45 |
C | |||||||
Culture fluid | 36.6 | 2.685 | 13.6 | 100 | 1.0 | ||
Ultrafiltration | 7.7 | 0.278 | 27.6 | 20.9 | 2.0 | ||
Mono Q PRMnP1 | 2.3 | 0.026 | 88.6 | 122 | 6.2 | 6.5 | 50 |
D | |||||||
Culture fluid | 52.0 | 4.669 | 11.2 | 100 | 1.0 | ||
Ultrafiltration | 24.6 | 2.713 | 9.1 | 47.2 | 0.8 | ||
Mono QPradMnP1 | 5.8 | 0.245 | 23.7 | 33 | 11.1 | 2.1 | 50 |
Mono Q PradMnP2 | 4.0 | 0.199 | 19.9 | 21 | 7.6 | 1.8 | 50 |
Mono Q PradMnP3 | 7.2 | 0.234 | 30.8 | 33 | 13.8 | 2.8 | 50 |
E | |||||||
Culture fluid | 7.1 | 0.671 | 10.6 | 100 | 1.0 | ||
Ultrafiltration | 3.4 | 0.511 | 6.6 | 46.9 | 0.6 | ||
Mono Q NFMnP1 | 0.7 | 0.012 | 55.2 | 67 | 9.3 | 5.2 | 49 |