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AMB Express

Table 2 Oligonucleotides used for the plasmid constructions

From: Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

Primers

                        Sequence (5’-3’)

thlp-for

GCATGCGAATTTAGAATGAAGTTTCTTATGCA

thlp_rev

AAAA GGGCCCCCATAGTTTATCCCTAATTTATACG

thlg_rev

CTCTGTAGAACTAGTTTATAATTCTACAGAGTTATTTTTAAC

s-adh-for

TTTT GGGCCCTTAGACTATTAAAGGAATATTTTTAAGG

s-adh-rev

TTTTCTCGAG GTATAATCCTCCATGATCTATTATG

ctfAB for

CAACTACTCGAGA TAATTTTTCT AG AGAATTTAAAAGGAGG GATTAAAATG

ctfAB rev

AATGGTACTAG TTATTTTTTGTCG AC TGTTTCATAGTATTTC TTTCTAAACAGCC

adc for

AAACAACTC GA GTTATAATC TAG ATATAAATAAATAGGA CTAGAGG CG

adc rev

AAAAATACT AGTTACCATTTAAGTC GACTCTTATTTTTATTA CTTAAG

  1. Bases not complementary with genomic DNA are in italics;
  2. Added restriction sites (ApaI, SalI, speI, SphI, XbaI and XhoI,) are underlined;
  3. Ribosome binding sites are in bold.
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