Figure 2From: Purification and properties of S-hydroxymethylglutathione dehydrogenase of Paecilomyces variotii no. 5, a formaldehyde-degrading fungusEffects of temperature on activity and stability of purified S -HMGSH dehydrogenase. The reaction was carried out under standard assay conditions described in the Materials and methods section, except for varying the temperature (open symbols). In experiments on stability, the enzyme was treated at various temperatures for 30 min in 50 mM phosphate buffer (pH 8.0) and the activity remaining was measured under standard assay conditions (closed symbols).Back to article page