Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168

AMB Express

Table 1 Assigned concentrations of different parameters and their levels in Plackett-Burman design for phytase production

Codes	Factors	Level (-1)	Level (+1)
A	T	30	37
B	pH	5.5	7.5
C	Shaking speed (rpm)	150	250
D	Inoculum size (OD600_f)	0.05	0.5
E	KH₂PO₄	0	5
F	Methanol	0	5
G	Glycerol	0	5
H	Galactose	0	5
I	Urea	0	5
J	Casein hydrolysate	0	5
K	Yeast extract	0	5
L	(NH₄)₂ SO₄	0	5
M	Triton X-100	0	5
N	Phytic acid	0	0.01
O	Corn steep liquor	0	10