Figure 2

Non-reducing SDS-PAGE of Cpl-1^C45S;D194C PEGylated with PEG 10K. Protein ladder (lanes 1 and 3); Cpl-1^C45S;D194C PEGylated with PEG 10K and purified on a DEAE-sepharose column (lane 2); further purification of Cpl-1^C45S;D194C PEGylated with PEG 10K on a Hiload 16/60 Superdex column (lane 4 to 12). Fractions 6, 7, and 8 were pooled and further used in the in vitro killing assay. Residual non-PEGylated Cpl-1^C45S;D194C is shown (lane 11 and 12).