Figure 1

Non-reducing SDS-PAGE of Cpl-1^C45S;D194C PEGylated with PEG 40K. Protein ladder (lanes 1 and 6); non-reduced Cpl-1^C45S;D194C (lane 2); Cpl-1^C45S;D194C reduced with 10 mM DTT before and after desalting on a PD-10 column (lane 3 and 4, respectively); Cpl-1^C45S;D194C PEGylated with PEG 40K and purified on a DEAE-sepharose column (lane 5); further purification of Cpl-1^C45S;D194C PEGylated with PEG 40K on a Hiload 16/60 Superdex column (lane 7 to 11). Fractions 9 and 10 were pooled and further used in the in vitro killing assay.