Table 2 Oligonucleotides used
Name | Sequence (5' to 3') | Description |
|---|---|---|
ON29 | GGCCATGGATCC ATGATTCAAAAACGAAAGCGGACAG | 5' end of amyQ |
ON42 | GGCCATGACGTC TTTCTGAACATAAATGGAGACGGAC | 3' end of amyQ |
ON47 | GGCCATGACGTC TTGGAAGCGACAGTTGAGTACG | 5' end of yhcR |
ON48 | GAATAAGATATC TCACGTTCTGGAGGCGCTCCT | 3' end of yhcR |
ON49 | GGCCATGACGTC CGCATGTTTGATATTGAAGAAGC | 5' end of yfkN |
ON50 | AGCAGCGATATC TTATGCCTGATTCGCTCTATTCTG | 3' end of yfkN |
ON54 | GGCCATTTCGAAGACCTCTTTAGCTCCTTGGAAGC | 3' end of erm |
ON55 | GACCTGAATGTGGAACGAGTGGAC | 5' end of ywpF |
ON56 | GGCCATTTCGAACCGACTGTAAAAAGTACAGTCGGCA | 3' end of cat |
ON57 | CGTCTTGATCAGGATACATCTGGC | 5' end of yhcT |
ON58 | GAGAGCCATAAACACCAATAGCCTT | 5' end of neo |
ON59 | GGCCATGAATTC AAAGGAGGAACTCCAGAACGTGAAAAAAGTTATTC | 5' end of yhcS |
ON60 | CTAATACGACTCACTATAGGGAGAGGATCC CGACACCTTTTTCTAAATCA | 3' end of yhcS |
ON61 | GGCCATGAATTC AAAGGAGGAACAACAATGCGCCGGGATCA | 5' end of ywpE |
ON62 | CTAATACGACTCACTATAGGGAGAGGATCC TCTTCGTGCTTCACTCTTGC | 3' end of ywpE |
ON63 | TCTACATCCAGAACAACCTCTGC | 5' end of Pspac |
ON64 | GGCCATAGATCT ATGCGCCGGGATCAAAAAATG | 5' end of ywpE |
ON65 | GGCCATAGATCT ATGAAAAAAGTTATTCCACTATTCATCATTGC | 5' end of yhcS |
ON66 | GGCCATAGATCT AGAATGAAGAAAAGCCGCAGGCACT | 3' end of yhcS |
ON67 | CCAGAGATCT CAAAGGAGGAACTCCAGAACGTGAAAAAAGTTATTC | 5' end of yhcS |
ON68 | AGTAAAGTTATCGGAATCGACTTAG | 5' end of dnaK |
ON69 | CTAATACGACTCACTATAGGGAGAAAAGTATGCAGGAACTGTGAT | 3' end of dnaK |