Phytosynthesis of BiVO4 nanorods using Hyphaene thebaica for diverse biomedical applications

Biosynthesis of bismuth vanadate (BiVO4) nanorods was performed using dried fruit extracts of Hyphaene thebaica as a cost effective reducing and stabilizing agent. XRD, DRS, FTIR, zeta potential, Raman, HR-SEM, HR-TEM, EDS and SAED were used to study the main physical properties while the biological properties were established by performing diverse assays. The zeta potential is reported as − 5.21 mV. FTIR indicated Bi–O and V–O vibrations at 640 cm−1 and 700 cm−1/1120 cm−1. Characteristic Raman modes were observed at 166 cm−1, 325 cm−1 and 787 cm−1. High resolution scanning and transmission electron micrographs revealed a rod like morphology of the BiVO4. Bacillus subtilis, Klebsiella pneumonia, Fusarium solani indicated highest susceptibility to the different doses of BiVO4 nanorods. Significant protein kinase inhibition is reported for BiVO4 nanorods which suggests their potential anticancer properties. The nanorods revealed good DPPH free radical scavenging potential (48%) at 400 µg/mL while total antioxidant capacity of 59.8 µg AAE/mg was revealed at 400 µg/mL. No antiviral activity is reported on sabin like polio virus. Overall excellent biological properties are reported. We have shown that green synthesis can replace well established processes for synthesizing BiVO4 nanorods.


Introduction
Phytosynthesis of nanoscaled materials is an innovative approach often considered as a potential replacement for various chemical or physical methods. The inherent nature of the chemical process often led to produce toxic wastes while the physical means are often accompanied with elevated energy requirements (Ovais et al. 2018b;Khalil et al. 2019a, b;Shah et al. 2018;Hassan et al. 2018). Relative to the biologically synthesized nanoparticles, the chemically synthesize nanoparticles indicate low biocompatibility and possess latent biological risks. In order to keep the energy balance and mitigating environmental risks, plants are used as a versatile bio-reductant for the synthesis of various metal nanoparticles or their nanocomposites. Medicinal plants possess a diverse reservoir of phytochemicals which can reduce and stabilize the nanoparticles (Ovais et al. 2018c;Mohamed et al. 2019). Metal and metal based nanomaterials have diverse applications in different fields and therefore a number of scientists have adopted novel methods for synthesis and application Thema et al. 2016;Devika et al. 2012;Mwakikunga et al. 2010;Khamlich et al. 2011).
Metal vanadate have been frequently looked for potential applications as implantable cardiac defibrillators, batteries, catalysis and photo catalysis (Sivakumar et al. 2015). However, bismuth vanadate has emerged as a promising candidate due to its unique physiochemical, optical and ferro-elastic properties (Sarkar and Chattopadhyay 2012). Various applications of BiVO 4 has been well studied in water splitting, sensors, pollutant degradation etc. (Ma et al. 2019;Vo et al. 2019;Prado et al. 2019;Jaihindh et al. 2019;Hassan et al. 2019;Chomkitichai et al. 2019). Recently, there has been growing interest in biological applications of BiVO 4 . The AgI-BiVO 4 composite material indicated excellent potential for inactivation of Escherichia coli in water disinfection (Guan et al. 2018). Similarly, the octahedral shaped BiVO 4 synthesized via hydrothermal approach revealed inactivation of E. coli (80% to 100%) (Sharma et al. 2016). 100% inactivation of E. coli after 30 min of exposure to Ag loaded BiVO 4 is also reported (Regmi et al. 2018). BiVO 4 is among few materials that remain stable in mild pH neutral conditions (Lichterman et al. 2013).
Due to the exciting properties and applications, there is considerable interest for the commercially scalable process for synthesizing BiVO 4 . Different methods like, ultrasonicassisted, hydrothermal, pyrolysis, flame spray, chemical bath deposition, sonochemical, template-free solution and co-precipitation method have been explored to synthesize BiVO 4 nanoparticles (Hu et al. 2018;Tao et al. 2019). Recently, plant extracts of Callistemon viminalis were used as a low cost reducing and stabilizing agents for biosynthesis of BiVO 4 (Mohamed et al. 2018). Complementing to the limited literature on green avenues and biological properties of BiVO 4 , a green method was adopted by using dried fruit aqueous extracts of Hyphaene thebaica as green scaffolds for the synthesis of rod shaped BiVO 4 which were subsequently studied for various biological properties. H. thebaica is a member of Arecaceae, locally referred as Doum (Arabic) and gingerbread tree (English). The medicinal applications of H. thebaica is well reported in the ethnomedicinal and folkloric scriptures (Khalil et al. 2019a, b). Various preparation of H. thebaica is reported for bleeding, haematuria, dyslipidemia, antihypertensive, diuretic diaphoretic, hypertension and lowering blood pressure etc. (Abdulazeez et al. 2019). In view of the medicinal applications and ethnopharmacological relevance, the fruit part of the H. thebaica was selected for biosynthesis.

Processing of plants
The fruits of H. thebaica were obtained from (Aswan) Egypt, gently washed in running distill water for removing dust/impurities or any form of particulate matter, shade dried, powdered and used for extraction by heating 10 g of powdered fruit material to 400 mL of distil water at 100 °C/2 h on magnetic stirrer hotplate. Residual wastes were removed by filtering extracts for three times with Whattman filter paper and the remaining transparent extracts were used further.

Biosynthesis of BiVO 4
Bismuth nitrate (2.448 g) was added to 50 mL aqueous extracts and heated at 100 °C/1 h for ensuring complete dissolution of precursor salt. In a separate flask, VOSO 4 (1.126 g) was introduced to 50 mL extracts and heated at 100 °C/1 h. Change in color was observed. Both solutions were mixed to make a mix of bismuth and vanadium ions, proportionally mixed to form bismuth vanadate. The resultant precipitates were washed three times by centrifugation and dried at 100 °C. The dried precipitate was annealed at 500 °C for 2 h in a tube furnace which yielded yellow colored powder assumed as BiVO 4 . Annealing was performed to obtain a high degree crystallinity and purity.

Physical properties
Diverse techniques were applied to elucidate the main physical properties of green synthesized BiVO 4 . Powder X-ray diffraction was carried with diffractometer equipped with an irradiation line of 1.5406 A 0 Cu Kα operating in Bragg-Brentano geometry. Debye Scherer formula was used to calculate the nano size while the data was compared with standard diffraction database. Vibrational characteristics were studied using Raman spectroscopy and FTIR. Diffuse reflectance spectra was recorded. Morphology was studied using HR-SEM and HR-TEM. Elemental composition was analyzed by Energy Dispersive Spectroscopy while Selected Area Electron Diffraction and zeta potential was also investigated. Once the physiochemical nature of the nanoparticles was established, they were then processed for analyzing their biomedical applications.

Antimicrobial properties
Simple well diffusion assay as described earlier (Khalil et al. 2014) was used at different concentration to investigate the antibacterial and antifungal potential of the BiVO 4 nanorods in the concentration range of 4 mg/mL to 250 µg/ mL.  (FCBP 434). Briefly, the microbial cultures were standardized to an optical density of 0.5, corresponding to the MacFarland standards. 100 µL of inocula was dispensed on the Tryptic Soy Agar (bacterial media) and Sabouraud Dextrose Agar (fungal media) plates which was uniformly spread with sterile cotton swabs. Through sterile borer, 5 mm wells were made and 30 µL samples was introduced. Erythromycin and Amp B were used as positive control for bacteria and fungi respectively, while DMSO was added as a negative control. The bacterial cultures were incubated at 37 °C for 24 h while the fungal plates were incubated at 37 °C for 72 h. Zones of inhibition was measured and the MIC was considered as the least test concentration to cause microbial inhibition.

Protein kinase inhibition
Streptomyces 85 E cultured on ISP4 medium was used to assess the PK inhibition as described previously (Fatima et al. 2015), from 4 mg/mL to 250 µg/mL. The standardized culture (100 µL) was dispensed on the media plates and spread uniformly. 5 mm borer was used to make wells and the test samples were introduced followed by incubation for 72 h at 30 °C. Bald and clear zones were measured while DMSO and Streptomycin were used as negative and positive controls respectively.

Antioxidant assays
DPPH free radical scavenging and total antioxidant capacity were performed in the concentration range of 400-25 µg/mL, through a spectrophotometer based method as described previously (Hameed et al. 2019). The DPPH reagent solution was prepared by dissolving DPPH (9.6 mg) in methanol (100 mL). Test samples (20 µL) was added to DPPH reagent (180 µL), and the incubated for 20 min in dark. Results were recorded at 517 nm, and calculations were performed according to; Total antioxidant capacity (Karunakaran et al. 2016) was investigated using phosphomolybdenum based method and the results were expressed as ascorbic acid equivalents per milligram.

Hemolysis
Hemolytic activity was performed as described previously (Malagoli 2007). Erythrocytes were isolated from freshly collected human blood in EDTA tubes, and their subsequent centrifugation at 14,000 RPM/5 min. 200 µL erythrocytes were added to 9.8 mL PBS for making erythrocytes suspension. The test nanoparticles in different concentrations were introduced in the Eppendorf tubes having an equal amount of the made erythrocytes suspension and incubated for 1 h at 35 °C. The reaction mix was then centrifuged at 10,000 RPM/10 min. Obtained supernatant was dispensed gently in 96 well plates and the hemoglobin release was monitored at 540 nm. Hemolysis was determined using the following formula;

Cell culture and antiviral experiments
Human Rhabdomyosarcoma Cells (RD), Human Laryngeal Carcinoma (HEp-2 cells) and L20B cells (mouse fibroblast cells) were enriched in Eagle's Minimal Essential Medium (E'MEM) containing (10%) FBS. Propagation of Sabin like Poliovirus (Type 1) was done through HEp-2 cells supplemented with 2% FBS. Viral titers were determined using Karber formula after titration of the virus on RD cell (Thuy et al. 2013).

Assessment of cytotoxicity
MTT assay was used for cytotoxicity assessment with slight modifications (Lin et al. 2005). MTT assay is based on the mitochondrial dehydrogenase of viable cells, giving blue formazan product quantified spectrophotometrically. MTT assay was performed in 96-well plates, seeded with 100 µL RD cells, HEp-2 cells and L20B cells at a concentration of 3.5 × 10 5 cells/mL cultured in E'MEM (200 μL) containing FBS (10%) and incubated at 36 °C for 48 h in CO 2 incubator to maintain a stable normal cell monolayers. Afterwards cells were treated with different doses of BiVO 4 NPs (1000-15 μg/mL), and incubated for an additional 48 h at 36 °C. Cells were examined daily under inverted light microscope to determine the minimum concentration of BiVO 4 NPs resulting in morphological changes in cells. 100 μL of MTT solution (5 mg/mL) was introduced to wells after removing the media and incubated (4 h/37 °C). MTT solution was then discarded and 50 μL dimethyl sulfoxide (DMSO) was added to dissolve insoluble formazan crystals and incubated (37 °C/30 min). Optical density (OD) was measured at 540 nm using a spectrophotometer reader (victor × 3, Perkin Elmer). Data were obtained from triplicate wells. Cell viability was expressed with respect to the absorbance of the control wells (untreated cells), which were considered as 100% of absorbance. The percentage of cytotoxicity is calculated as where A and B are the OD540 of untreated and of treated cells, respectively. The 50% cytotoxic concentration (CC50) was defined as the compound's concentration (μg/mL) required for the reduction of cell viability by 50%.

Assessment of antiviral activity
Confluent RD, Hep2C and L20B cell culture were treated with mixture of BiVO 4 NPs and virus dilutions. Firstly, 100TCID 50 poliovirus type 1 were diluted tenfold into two concentration of 10TCID 50 and 1TCID 50 in 2% E'MEM and introduce to non-cytotoxic concentrations of BiVO 4 NPs (15 µg/mL) in ratio of 1:1 (v/v) and incubated for 1 h at 36 °C . After that, mixture of virus dilutions (100TCID, 10TCID50 and 1TCID 50 ) was incubated with BiVO 4 NPs (1 mg/mL to 15 µg/mL) in 96 well plate seeded with healthy monolayer of Hep2C cells (3.5 × 10 5 cells/mL) in a CO 2 incubator with 5% CO 2 . Cell growth 10% EMEM medium was decanted and replaced with 200 µL media respectively. Three controls were used including: (i) 50 μL of BiVO 4 NPs at 15 µg/mL concentration (without poliovirus) were added to wells containing RD cells for BiVO 4 NPs control (Magudieshwaran et al. 2019); 50 µL of poliovirus at concentration of 1TCID 50 , 10TCID 50 and 100TCID 50 was added to wells (iii) 200 μL of fresh maintenance medium was added for negative controls. The cultures were incubated at 36 °C post-infection, and cytopathic effect (CPE) was daily observed by inverted light microscopy. The cellular viability was determined through staining method using crystal violet. Optical density (OD) was measured at 490 nm using a spectrophotometer.

Physical characterizations
Hyphaene thebaica dried fruit aqueous extracts were used as bio reductant for synthesis of novel BiVO 4 nanorods. Different techniques were used to characterize the room temperature physiochemical properties of the nanorods. The overall process and study scheme has been summarized in Fig. 1. The extracts were treated separately with precursor salts of bismuth and vanadium giving light brown and blue color. Powdered X-ray diffraction was carried out to reveal the crystallographic properties and presence of BiVO 4 nanorods. Figure (321) and (123) respectively. These crystallographic peaks were in correspondence with the JCPDS pattern 00-014-0688 for Clinobisvanite phase monoclinic bismuth vanadium oxide (BiVO 4 ). Sharpness of the peaks indicate a highly crystalline nature of the BiVO 4 . No other peaks were detected which suggested the single phase purity of the BiVO 4 nanorods. The crystal structure belonged to space group I2/a with lattice parameters were deduced as 〈a〉 = 5.1 A°, 〈b〉 = 11.7 A°, and 〈c〉 = 5.09 A° correlating to the BiVO 4 with yellow color. Scherer approximation revealed average size of ~ 7 nm as indicated in Table 1A.
After establishing single phase purity of BiVO 4 nanorods, their elemental analysis were carried out using Energy Dispersive Spectroscopy as indicated in Fig. 2b. Spectral analysis confirmed the presence of "Bi", "V" and "O". The peak of "C" relates to the grid support. Some traces of "Cu" and "K" were also found that most probably emanates from the organic components of the fruit material. Figure 2c indicate the FTIR spectra of the biosynthesized BiVO 4 nanorods from 200 to 4000 cm −1 . Main absorption peaks were observed centered at ~ 640 cm −1 , ~ 700 cm −1 , ~ 1120 cm −1 , ~ 1625 cm −1 and 3400 cm −1 . Peak centered at ~ 640 cm −1 can be ascribed to Bi-O (bending) while at ~ 700 cm −1 and ~ 1120 cm −1 to V-O symmetric and asymmetric vibrations (Khan et al. 2017). IR peaks centered at ~ 1625 cm −1 and 3400 cm −1 can be ascribed to the stretching vibrations of O-H group.
Raman spectroscopy is considered as a powerful technique to probe structure of metal oxides. Raman spectroscopy was carried out to further elaborate the vibrational properties of BiVO 4 nanorods in the spectral range of 0 cm −1 to 1500 cm −1 . Three noticeable raman peaks were observed centered at 166 cm −1 , 325 cm −1 and 787 cm −1 . The intense stretching mode of VO 4 is observed at 787 cm −1 . Raman peak centered at 325 cm −1 represents the asymmetric bending mode of VO 4 tetrahedron (Brack et al. 2015). Peak centered at 166 cm −1 , is attributed to the external mode vibration (Xu et al. 2018;Nikam and Joshi 2016). The raman spectra of BiVO 4 nanorods is indicated in Fig. 2d. The UV-Vis diffuse reflectance spectrum was recorded from 0 to 3000 nm. The BiVO 4 nanorods revealed good visible light absorption with absorption edge at 487 nm. The steep shape is ascribed to the band gap transitions. The energy of the band gap is estimated to be ~ 2.54 eV. DRS spectra has been indicated in Fig. 3.
Inset Fig. 4A-F indicate the various high resolution microscopic images of the synthesized nanoparticles to establish their morphology. One can conclude the formation of well aligned rod shape of the BiVO 4 . The Selected Area Electron Diffraction pattern suggest crystalline nature of the nanorods as indicated in Fig. 4F. Zeta potential of the BiVO 4 nanorods was recorded as − 5.21 mV. Results are indicated in Table 1B.

Antimicrobial properties
The antimicrobial properties of the BiVO 4 nanorods have been explored against various bacterial and fungal strains. Results of the antibacterial and antifungal properties are indicated in Fig. 5a, b. Among the tested bacterial strains, B. subtilis revealed highest zone of inhibition (20 mm to 9.5 mm) in the concentration range of 4 mg/ mL to 250 µg/mL. The least susceptible strain was found to be E. coli which revealed maximum zone of inhibition (11.5 mm) at 4 mg/mL. The order of the antibacterial activity of BiVO 4 nanorods was found as B. subtilis > K. pneumoniae > S. epidermidis > P. aeruginosa > E. coli. Interestingly for P. aeruginosa and S. epidermidis, the observed zone of inhibition was much larger than the positive control Erythromycin at the rate of 1 mg/mL. Similarly, against K. pneumoniae, the BiVO 4 nanorods were found to be as effective as the positive control. Among the five tested fungal strains, F. solani was observed as most susceptible fungal strain revealing zones ranging from 13 to 5.7 mm at the tested concentrations of BiVO 4 nanorods. The order by which the antifungal potential observed was F. solani > A. niger > Mucor sp. > A. fumigatus > A. flavus. A. niger did not revealed any zones at 500 µg/mL or below, while A. fumigatus was found to be in effective at 250 µg/mL. Against F. solani and A. niger, the zones were revealed similar to the zones obtained from positive control (Amp B). Results of antifungal activity are summarized in Fig. 5b. Figure 6 indicate various selected images of the antibacterial and antifungal activities. Moreover, these activities revealed a dose dependent response.

Protein kinase inhibition
A simple assay based on the Streptomyces 85 E strain is used to screen PK inhibitors. Figure 7a, b indicate the protein kinase inhibition potential of the H. thebaica mediated BiVO 4 nanorods. Excellent PK inhibition was revealed. The zones of inhibition at the tested concentration ranged from 13 to 8 mm. However, the zones of inhibition was much smaller then obtained for positive control.

Antioxidant assays
The antioxidant potential of the BiVO 4 nanorods was determined using DPPH free radical scavenging and total antioxidant capacity. Moderate free radical scavenging potential is reported. At the highest tested concentration of 400 µg/mL, the percent scavenging was found to be 48%, which gradually declined as the concentration was lowered below 400 µg/mL. At the lowest tested concentration (25 µg/mL) of BiVO 4 nanorods, 29% scavenging was observed. These results were complemented by the total antioxidant capacity which was determined as µg AAE/mg. Highest value (59.8 µg AAE/mg) of the ascorbic acid equivalents (AAE) was reported at 400 µg/mL while at lowest concentrations of 25 µg/mL, 26.9 µg AAE/mg was reported. Overall the antioxidant activity can be concluded as moderate and dose dependent. Results of antioxidant potential is indicated in Fig. 7c.

Hemolysis
Erythrocytes lysis assay was performed to evaluate the toxicity of BiVO 4 on fresh isolated RBCs in test concentrations ranging from 600 to 12.5 µg/mL. The BiVO 4 nanorods were observed to cause increased degree of hemolysis (75%) at higher concentrations 600 µg/ mL, while percent hemolytic potential decreased with decrease in concentration. At lowest tested concentration

Antiviral activity of BiVO 4
In order to investigate the antiviral activity of BiVO 4 , three concentrations (1TCID 50 , 10TCID 50 and 100TCID 50 ) of Sabin like poliovirus (Type 1) were incubated with BiVO 4 NPs (15 µg/mL). Our results indicated that cells remained viable at 24 h post-infection. At 5th day of incubation, it was observed that most Hep2C cells were destroyed at viral concentration of 100TCID50, 10TCID 50 and 1TCID 50 across all the tested concentrations of BiVO 4 NPs. It can be inferred that the BiVO 4 nanorods were unable to inhibit the propagation of polio virus in the Hep2C cells. Complete destruction of the Hep2C cells was due to the intracellular propagation of the polio virus in Hep2C, cultured with 15 µg/mL of the BiVO 4 nanorods.

Discussion
The interface of green nanotechnologies and medicinal plants have delivered excellent results over the previous decades. A number of biogenic metal based nanoparticles has revealed excellent results (Sathiyavimal et al. 2018). Green synthesized nanoparticles often exhibit multifunctional nature and therefore can be applied in diverse applications (Nasar et al. 2019;Venugopal et al. 2017). The interesting properties and potential applications of BiVO 4 has fueled the growing research on their synthesis procedures which easy, scalable, green and cost effective. Different chemical and physical processes have been adopted for the synthesis of BiVO 4 , however, the potential of biological resources in their synthesis is largely untapped. Recently, we have established the successful synthesis of BiVO 4 by using Callistemon viminalis floral extracts as bioreductant (Mohamed et al. 2018). Herein, a further detailed study was conducted on the physical as well as biological properties of BiVO 4 nanorods, synthesized using the fruit extracts of H. thebaica. Plant extracts are reported to have a rich chemistry which has the tendency to catalyze redox reactions and subsequently stabilize the nanoparticles. The phytochemicals that usually take part in the reduction are mostly considered to be phenols, flavonoids, citric acid, membrane proteins, reductases, dehydrogenases etc. while the stabilizing moieties can be tannic acids, extracellular proteins, peptides, enzymes (Karatoprak et al. 2017;Akhtar et al. 2013;Elegbede et al. 2018 (Gawande and Thakare 2012;Sivakumar et al. 2015). In and Clinobisvanite (monoclinic). Among these mineral forms, Clinobisvanite is most stable thermodynamically and possess significant photocatalytic potential (Zhao et al. 2011). Depending on the conditions, ferroelastic monoclonal-tetragonal-phase transitions are reported (Frost et al. 2006). The elemental analysis confirms the presence of "Bi", "V" and "O" which establishes the synthesis of BiVO 4 . The infrared spectra of the synthesized nanorods affirms the potential role of phenolic components in plant extracts that have catalyzed the reduction and stabilization of BiVO 4 nanorods. The role of phenolic compounds as reducing agents is well established (Ovais To date, most of the studies revealing the antimicrobial potential of BiVO 4 considered only the waste water disinfection. Recently, an innovative photocatalytic fabricated by Ni doping on BiVO 4 revealed excellent degradation of ibuprofen (80%) within 90 min, while 92% reduction of E. coli after 5 h exposure to light was recorded. In addition, Ni-BiVO 4 indicated excellent anti algal potential (Regmi et al. 2017). A novel BiVO 4 /InVO 4 nanocomposite material revealed excellent sterilization potential against various bacterial strains i.e. E. coli (99.71%), S. aureus (99.55%), P. aeruginosa (99.54%) and A. carterae (96%) (Zhang et al. 2019). In a recent report, graphene based nanocomposite of BiVO 4 (90 mg/L) was studied for antibacterial potential against B. subtilis and S. aureus using disc diffusion assay with, but no zone of inhibition was observed, suggesting a nontoxic nature of the BiVO 4 -GO nanocomposite (Zhao et al. 2019). Our work describe for the first time the antimicrobial potential of the phytosynthesized BiVO 4 . The physiochemical nature of the nanorods (surface coating, reducing-stabilizing agents, shape, size, surface morphology) plays important role in determining the antimicrobial activities (Zhang et al. 2016). The mechanism that drives the antimicrobial potential of the metal nanoparticles has mostly been attributed to the generation of reactive oxygen species. The present age of antibiotic resistance signifies the need to develop alternative antibiotics. The microorganisms tends to smartly evolve in order to develop resistance to the available treatments at a speedy rate. Furthermore, new antibiotics are not produced at the same pace at which microorganisms are getting resistant. Novel approaches like nanoantibiotics are considered vital to curb antibiotic resistance. BiVO 4 nanorods have indicated excellent antimicrobial activities and therefore can be considered as a novel nanoantibiotics for future, before detailed evaluation of toxicity. The inhibition of protein kinase enzymes is considered a popular target for the anticancer therapies. Therefore, tremendous research has been devoted for identifying potent inhibitors of PK enzymes. Protein kinase are responsible for phosphorylating serine-threonine and tyrosine amino acids and play integral role in signaling differentiation and division of cells. The malfunctioned phosphorylation leads to the progression of cancer. By inhibiting the protein kinase that serve as a bridge for the signaling factors, cell division can be stopped ultimately hindering cancer progression. PK enzymes are vital for the growth of hyphae in Streptomyces 85 E strain and therefore, considered as a model organism. The cell culture experiments suggested the viability of the cells at low concentrations of the BiVO 4 nanorods.
With the advances of the metal nanoparticles research medicinal plants have emerged as an exciting resource to be explored from green synthesis aspects. We have reported the biosynthesis of BiVO 4 nanorods using H. thebaica fruit extracts as a low cost and green templating agents and studied them for possible biological applications. Excellent antibacterial and antifungal activities are reported. BiVO 4 nanorods were most effective on Bacillus subtilis and Fusarium solani. Good protein kinase inhibition and antioxidant potential is revealed. The BiVO 4 induced hemolysis at high concentrations. At low concentrations, the cell culture experiments revealed compatibility and non-toxicity. No potential antiviral activity was identified for BiVO 4 .
Green synthesis using medicinal plant extracts provides an excellent platform for assembling nanomaterials for different applications. The process is not only economical but converging evidence suggests enhanced compatibility of the biosynthesized nanoparticles making them ideal for nanomedicinal applications. Most of the work in this area has been dedicated to the silver and gold nanoparticles and their nanomedicinal applications which necessitates the need of extending this methodology to novel nanomaterials. BiVO 4 has diverse applications in industries and further research is encouraged to use to different plant extracts to synthesize BiVO 4 and explore their biomedical potential.