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  1. Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabino...

    Authors: Bala Muntari, Azura Amid, Maizirwan Mel, Mohammed S Jami and Hamzah M Salleh
    Citation: AMB Express 2012 2:12
  2. Optically pure β-amino acids constitute interesting building blocks for peptidomimetics and a great variety of pharmaceutically important compounds. Their efficient synthesis still poses a major challenge. Tra...

    Authors: Jens Rudat, Birgit R Brucher and Christoph Syldatk
    Citation: AMB Express 2012 2:11
  3. To attempt cost-effective production of US417 phytase in Bacillus subtilis, we developed an efficient system for its large-scale production in the generally recognized as safe microorganism B. subtilis 168. Hence...

    Authors: Ameny Farhat-Khemakhem, Mounira Ben Farhat, Ines Boukhris, Wacim Bejar, Kameleddine Bouchaala, Radhouane Kammoun, Emmanuelle Maguin, Samir Bejar and Hichem Chouayekh
    Citation: AMB Express 2012 2:10
  4. Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed ba...

    Authors: Ryoich Nakamura, Ryuichiro Kondo, Ming-hao Shen, Hideharu Ochiai, Shin Hisamatsu and Shigenori Sonoki
    Citation: AMB Express 2012 2:8
  5. The capacity of white-rot fungi to degrade wood lignin may be highly applicable to the development of novel bioreactor systems, but the mechanisms underlying this function are not yet fully understood. Lignin ...

    Authors: Takaiku Sakamoto, Yuki Yao, Yoshifumi Hida, Yoichi Honda, Takashi Watanabe, Wataru Hashigaya, Kazumi Suzuki and Toshikazu Irie
    Citation: AMB Express 2012 2:7
  6. Large volumes of treated process water are required for protein extraction. Evaporation of this water contributes greatly to the energy consumed in enriching protein products. Thin stillage remaining from etha...

    Authors: Kornsulee Ratanapariyanuch, Robert T Tyler, Youn Young Shim and Martin JT Reaney
    Citation: AMB Express 2012 2:5
  7. The gene for a eukaryotic phenolic acid decarboxylase of Candida guilliermondii was cloned, sequenced, and expressed in Escherichia coli for the first time. The structural gene contained an open reading frame of ...

    Authors: Hui-Kai Huang, Li-Fan Chen, Masamichi Tokashiki, Tadahiro Ozawa, Toki Taira and Susumu Ito
    Citation: AMB Express 2012 2:4
  8. Analysis of the simocyclinone biosynthesis (sim) gene cluster of Streptomyces antibioticus Tü6040 led to the identification of a putative pathway specific regulatory gene simReg1. In silico analysis places the Si...

    Authors: Liliya Horbal, Yuriy Rebets, Mariya Rabyk, Roman Makitrynskyy, Andriy Luzhetskyy, Victor Fedorenko and Andreas Bechthold
    Citation: AMB Express 2012 2:1
  9. Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence fr...

    Authors: Georgiana F da Cruz, Suzan P de Vasconcellos, Célio FF Angolini, Bruna M Dellagnezze, Isabel NS Garcia, Valéria M de Oliveira, Eugenio V dos Santos Neto and Anita J Marsaioli
    Citation: AMB Express 2011 1:47
  10. Agrobacterium tumefaciens-mediated transformation (AMT) was applied to Aspergillus aculeatus. Transformants carrying the T-DNA from a binary vector pBIG2RHPH2 were sufficiently mitotically stable to allow functi...

    Authors: Emi Kunitake, Shuji Tani, Jun-ichi Sumitani and Takashi Kawaguchi
    Citation: AMB Express 2011 1:46
  11. Systems Biology has taken advantage of computational tools and high-throughput experimental data to model several biological processes. These include signaling, gene regulatory, and metabolic networks. However...

    Authors: Daniel Machado, Rafael S Costa, Miguel Rocha, Eugénio C Ferreira, Bruce Tidor and Isabel Rocha
    Citation: AMB Express 2011 1:45
  12. The use of biodegradable plastics can reduce the accumulation of environmentally persistent plastic wastes. The rate of degradation of biodegradable plastics depends on environmental conditions and is highly v...

    Authors: Hiroko K Kitamoto, Yukiko Shinozaki, Xiao-hong Cao, Tomotake Morita, Masaaki Konishi, Kanako Tago, Hideyuki Kajiwara, Motoo Koitabashi, Shigenobu Yoshida, Takashi Watanabe, Yuka Sameshima-Yamashita, Toshiaki Nakajima-Kambe and Seiya Tsushima
    Citation: AMB Express 2011 1:44
  13. We screened various thermophiles for meso-diaminopimelate dehydrogenase (meso-DAPDH, EC 1.4.1.16), which catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate, and found the enzyme in a the...

    Authors: Hironaga Akita, Yasuhiro Fujino, Katsumi Doi and Toshihisa Ohshima
    Citation: AMB Express 2011 1:43
  14. Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay ...

    Authors: Asha A Kulkarni-Almeida, Manoja K Brahma, Prabhu Padmanabhan, Prabhu D Mishra, Rajashri R Parab, Nitin V Gaikwad, Chandni S Thakkar, Pradipta Tokdar, Prafull V Ranadive, Amrutha S Nair, Anagha A Damre, Umakant A Bahirat, Nitin J Deshmukh, Lalit S Doshi, Amol V Dixit, Saji D George…
    Citation: AMB Express 2011 1:42
  15. The microbes in the gastrointestinal (GI) tract are of high importance for the health of the host. In this study, Roche 454 pyrosequencing was applied to a pooled set of different 16S rRNA gene amplicons obtai...

    Authors: Maartje AHJ van Kessel, Bas E Dutilh, Kornelia Neveling, Michael P Kwint, Joris A Veltman, Gert Flik, Mike SM Jetten, Peter HM Klaren and Huub JM Op den Camp
    Citation: AMB Express 2011 1:41
  16. A major problem for manufacturers of cracked spores Ganoderma lucidum, a traditional functional food/Chinese medicine (TCM), is to ensure that raw materials are consistent as received from the producer. To addres...

    Authors: Chee Wei Lim, Sheot Harn Chan and Angelo Visconti
    Citation: AMB Express 2011 1:40
  17. With the emergence and spread of multidrug resistant bacteria, effective methods to eliminate both planktonic bacteria and those embedded in surface-attached biofilms are needed. Electric currents at μA-mA/cm2 ra...

    Authors: Robert Szkotak, Tagbo H R Niepa, Nikhil Jawrani, Jeremy L Gilbert, Marcus B Jones and Dacheng Ren
    Citation: AMB Express 2011 1:39
  18. In this work, Lactobacillus reuteri has been metabolically engineered for improving 1, 3-propanediol (1, 3-PD) production by the expression of an Escherichia coli alcohol dehydrogenase, yqhD, that is known to eff...

    Authors: Hema Vaidyanathan, Vijayalakshmi Kandasamy, Gopi Gopal Ramakrishnan, KB Ramachandran, Guhan Jayaraman and Subramanian Ramalingam
    Citation: AMB Express 2011 1:37
  19. In winemaking, nutrient supplementation is a common practice for optimising fermentation and producing quality wine. Nutritionally suboptimal grape juices are often enriched with nutrients in order to manipula...

    Authors: Gal Winter, Paul A Henschke, Vincent J Higgins, Maurizio Ugliano and Chris D Curtin
    Citation: AMB Express 2011 1:36
  20. The upper parts of oil field structures may leak gas which is supposed to be indirectly detected by the soil bacterial populations. Such microorganisms are capable of consuming this gas, supporting the Microbi...

    Authors: Paula B Miqueletto, Fernando D Andreote, Armando CF Dias, Justo C Ferreira, Eugênio V dos Santos Neto and Valéria M de Oliveira
    Citation: AMB Express 2011 1:35
  21. Hydrophobicity is a very important surface property and there is a growing interest in the production and characterization of superhydrophobic surfaces. Accordingly, it was recently shown how to obtain a super...

    Authors: Cláudia Sousa, Diana Rodrigues, Rosário Oliveira, Wenlong Song, João F Mano and Joana Azeredo
    Citation: AMB Express 2011 1:34
  22. There is a need to elucidate the product specific features of the metabolic stress response of the host cell to the induction of recombinant protein synthesis. For this, the method of choice is transcriptomic ...

    Authors: Ashish K Sharma, Shubhashree Mahalik, Chaitali Ghosh, Anuradha B Singh and Krishna J Mukherjee
    Citation: AMB Express 2011 1:33
  23. Bovine viral diarrhea virus is a pestivirus in the family Flaviviridae that cause abortions and stillbirths in livestock and its traditional diagnosis is based on cell culture and virus neutralization test. In...

    Authors: Farhad Safarpoor Dehkordi
    Citation: AMB Express 2011 1:32
  24. An extracellular peroxygenase from Marasmius rotula was produced in liquid culture, chromatographically purified and partially characterized. This is the third aromatic peroxygenase (APO) that has been characteri...

    Authors: Glenn Gröbe, René Ullrich, Marek J Pecyna, Danuta Kapturska, Stephanie Friedrich, Martin Hofrichter and Katrin Scheibner
    Citation: AMB Express 2011 1:31
  25. Cellulose degradation is one of the major bottlenecks of a consolidated bioprocess that employs cellulolytic bacterial cells as catalysts to produce biofuels from cellulosic biomass. In this study, we investig...

    Authors: Zhi-Wu Wang, Seung-Hwan Lee, James G Elkins and Jennifer L Morrell-Falvey
    Citation: AMB Express 2011 1:30
  26. Methanogen populations and their domains are poorly understood; however, in recent years, research on this topic has emerged. The relevance of this field has also been enhanced by the growing economic interest...

    Authors: Deborah Traversi, Silvia Villa, Marco Acri, Biancamaria Pietrangeli, Raffaella Degan and Giorgio Gilli
    Citation: AMB Express 2011 1:28
  27. Six protease fractions, namely FI, FII, FIII-1, FIII-2, FIII-3 and FIV, were isolated from Perionyx excavatus earthworm biomass by acetone precipitation, followed by serial chromatography using anion exchange, hy...

    Authors: Tram Thi Bich Phan, Tien Duy Ta, Dung Thi Xuan Nguyen, Lambertus AM Van Den Broek and Giang Thi Huong Duong
    Citation: AMB Express 2011 1:26
  28. Effective utilization of photosynthetic microorganisms as potential biocatalysts is favorable for the production of useful biomaterials and the reduction of atmospheric CO2. For example, biocatalytic transformati...

    Authors: Rio Yamanaka, Kaoru Nakamura and Akio Murakami
    Citation: AMB Express 2011 1:24
  29. Soybean seeds are non-sterile and their bacterial population interferes with the enumeration of beneficial bacteria, making it difficult to assess survival under different conditions. Within this context, the ...

    Authors: Claudio Penna, Rosana Massa, Florencia Olivieri, Gabriel Gutkind and Fabricio Cassán
    Citation: AMB Express 2011 1:21
  30. Kluyveromyces marxianus possesses a useful potential to assimilate a wide variety of substrates at a high temperature, but the negative effect by coexisting glucose is critical for utilization of biomass contain...

    Authors: Noppon Lertwattanasakul, Nadchanok Rodrussamee, Suprayogi, Savitree Limtong, Pornthap Thanonkeo, Tomoyuki Kosaka and Mamoru Yamada
    Citation: AMB Express 2011 1:20
  31. Batch anaerobic digestion experiments using dairy manure as feedstocks were performed at moderate (25°C), mesophilic (37°C), and thermophilic (52.5°C) temperatures to understand E. coli, an indicator organism for...

    Authors: Pramod K Pandey and Michelle L Soupir
    Citation: AMB Express 2011 1:18
  32. The enhanced global biodiesel production is also yielding increased quantities of glycerol as main coproduct. An effective application of glycerol, for example, as low-cost substrate for microbial growth in in...

    Authors: Chlud Kaddor and Alexander Steinbüchel
    Citation: AMB Express 2011 1:16
  33. Enzymatic hydrolysis is one of the most important processes in bioethanol production from lignocellulosic biomass. Acremonium cellulolyticus is a filamentous fungus with high cellulase production but productivity...

    Authors: Machi Kanna, Shinichi Yano, Hiroyuki Inoue, Tatsuya Fujii and Shigeki Sawayama
    Citation: AMB Express 2011 1:15
  34. Azotobacter chroococcum is a widespread free-living soil bacterium within the genus of Azotobacter known for assimilation of atmospheric nitrogen and subsequent conversion into nitrogenous compounds, which hence...

    Authors: Susanne Herter, Marlen Schmidt, Mark L Thompson, Annett Mikolasch and Frieder Schauer
    Citation: AMB Express 2011 1:14
  35. Three different Baeyer-Villiger monooxygenases (BVMOs) were reported to be involved in the camphor metabolism by Pseudomonas putida NCIMB 10007. During (+)-camphor degradation, 2,5-diketocamphane is formed servin...

    Authors: Maria Kadow, Stefan Saß, Marlen Schmidt and Uwe T Bornscheuer
    Citation: AMB Express 2011 1:13
  36. The amino alcohol serinol (2-amino-1,3-propanediol) has become a common intermediate for several chemical processes. Since the 1940s serinol was used as precursor for synthesis of synthetic antibiotics (chlora...

    Authors: Björn Andreeßen and Alexander Steinbüchel
    Citation: AMB Express 2011 1:12
  37. Polyhydroxyalkanoates (PHAs) are biopolymers, which can replace petrochemical plastics in many applications. However, these bioplastics are currently far more expensive than petrochemical plastics. Many resear...

    Authors: Rob AJ Verlinden, David J Hill, Melvin A Kenward, Craig D Williams, Zofia Piotrowska-Seget and Iza K Radecka
    Citation: AMB Express 2011 1:11

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