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  1. The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in t...

    Authors: Rachid Bel-Rhlid, Dinesh Thapa, Karin Kraehenbuehl, Carl Erik Hansen and Lutz Fischer
    Citation: AMB Express 2013 3:28
  2. Current problems of filamentous fungi fermentations and their further successful developments as microbial cell factories are dependent on control fungal morphology. In this connection, this work explored new ...

    Authors: Verónica Leticia Colin, Mario Domingo Baigorí and Licia María Pera
    Citation: AMB Express 2013 3:27
  3. Long chain polyunsaturated fatty acids (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are considered essential omega-3 fatty acids in human nutrition. In marine microalgae EPA an...

    Authors: Peter Boelen, Roechama van Dijk, Jaap S Sinninghe Damsté, W Irene C Rijpstra and Anita GJ Buma
    Citation: AMB Express 2013 3:26
  4. In the last few years, synthesis of carrier-free immobilized biocatalysts by cross-linking of enzyme aggregates has appeared as a promising technique. Cross-linked enzyme aggregates (CLEAs) present several int...

    Authors: María del Pilar Guauque Torres, María Laura Foresti and María Luján Ferreira
    Citation: AMB Express 2013 3:25
  5. Streptomyces griseus DSM 2608 produces bafilomycin, an antifungal plecomacrolide antibiotic. We cloned and sequenced an 87.4-kb region, including a polyketide synthase (PKS) region, methoxymalonate genes, flaven...

    Authors: Jae Yoon Hwang, Hyo Sun Kim, Soo Hee Kim, Hye Ryeung Oh and Doo Hyun Nam
    Citation: AMB Express 2013 3:24
  6. Debaryomyces nepalensis, osmotolerant yeast isolated from rotten apple, is known to utilize both hexoses and pentoses and produce industrially important metabolites like ethanol, xylitol and arabitol. In the pre...

    Authors: Himabindu Kumdam, Shweta Narayana Murthy and Sathyanarayana N Gummadi
    Citation: AMB Express 2013 3:23
  7. Agricultural sustainability may represent the greatest encumbrance to increasing food production. On the other hand, as a component of sustainability, replacement of chemical fertilizers by bio-fertilizers has...

    Authors: Bettina Berquó Marks, Manuel Megías, Marco Antonio Nogueira and Mariangela Hungria
    Citation: AMB Express 2013 3:21
  8. To enhance the value-added use of methanol-containing raw glycerol derived from biodiesel fuel production, the effect of methanol supplementation on glyceric acid (GA) production by Gluconobacter spp. was investi...

    Authors: Shun Sato, Naoki Morita, Dai Kitamoto, Toshiharu Yakushi, Kazunobu Matsushita and Hiroshi Habe
    Citation: AMB Express 2013 3:20
  9. A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH ...

    Authors: Teppei Arai, Kasumi Koganei, Sara Umemura, Ryo Kojima, Jun Kato, Takafumi Kasumi and Jun Ogihara
    Citation: AMB Express 2013 3:19
  10. A cylindrical bioelectrochemical reactor (BER) containing carbon fiber textiles (CFT; BER + CFT) has characteristics of bioelectrochemical and packed-bed systems. In this study, utility of a cylindrical BER + ...

    Authors: Daisuke Sasaki, Kengo Sasaki, Atsushi Watanabe, Masahiko Morita, Yasuo Igarashi and Naoya Ohmura
    Citation: AMB Express 2013 3:17
  11. This report provides for the first time a novel environment friendly extracellular synthesis of stable silver nano-bioconjugates (SNBCs) at room temperature at pH 5.0 using Penicillium citrinum MTCC 9999 biomass....

    Authors: Achintya Mohan Goswami, Tuhin Subhra Sarkar and Sanjay Ghosh
    Citation: AMB Express 2013 3:16
  12. After steam pretreatment of lignocellulosic substrates the fermentation of the biomass derived sugars to ethanol is typically problematic because of both the generally low sugar concentrations that can be supp...

    Authors: Nuwan Sella Kapu, Maya Piddocke and Jack (John) N Saddler
    Citation: AMB Express 2013 3:15
  13. Peroxidases are oxidoreductase enzymes produced by most organisms. In this study, a peroxidase was purified from Hevea brasiliensis cell suspension by using anion exchange chromatography (DEAE-Sepharose), affinit...

    Authors: Thitikorn Chanwun, Nisaporn Muhamad, Nion Chirapongsatonkul and Nunta Churngchow
    Citation: AMB Express 2013 3:14
  14. A library of synthetic promoters of various strengths, specifically constructed for Streptomyces species, was cloned in the promoter-probe plasmid pIJ487, upstream of the promoter-less aphII gene that confers res...

    Authors: Nicolas Seghezzi, Marie-Joelle Virolle and Patrick Amar
    Citation: AMB Express 2013 3:13
  15. The aim of this research is to optimize the cultural conditions for the conversion of glycerol to ethanol by Enterobacter aerogenes S012. Taguchi method was used to screen the cultural conditions based on their s...

    Authors: Raymond E S Nwachukwu, Abolghasem Shahbazi, Lijun Wang, Mulumebet Worku, Salam Ibrahim and Keith Schimmel
    Citation: AMB Express 2013 3:12
  16. Silk-elastin-like polymers (SELPs) are protein-based polymers composed of repetitive amino acid sequence motifs found in silk fibroin (GAGAGS) and mammalian elastin (VPGVG). These polymers are of much interest...

    Authors: Raul Machado, João Azevedo-Silva, Cristina Correia, Tony Collins, Francisco Javier Arias, Jose Carlos Rodríguez-Cabello and Margarida Casal
    Citation: AMB Express 2013 3:11
  17. The extensive diversity of microalgae provides an opportunity to undertake bioprospecting for species possessing features suited to commercial scale cultivation. The outdoor cultivation of microalgae is subjec...

    Authors: Holly D Smith-Bädorf, Christopher J Chuck, Kirsty R Mokebo, Heather MacDonald, Matthew G Davidson and Rod J Scott
    Citation: AMB Express 2013 3:9
  18. 2,5-diketo-D-gluconic acid reductase (2,5-DKG reductase) catalyses the reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG), a direct precursor (lactone) of L-ascorbic acid (vitam...

    Authors: Vanja Kaswurm, Tien-Thanh Nguyen, Thomas Maischberger, Klaus D Kulbe and Herbert Michlmayr
    Citation: AMB Express 2013 3:7
  19. The repressor protein PhaR, which is a component of poly[(R)-3-hydroxybutyrate] granules, functions as a repressor of the gene expression of the phasin PhaP and of PhaR itself. We used a quartz crystal microbalan...

    Authors: Miwa Yamada, Shuntaro Takahashi, Yoshio Okahata, Yoshiharu Doi and Keiji Numata
    Citation: AMB Express 2013 3:6
  20. Auxotrophic mutants of Aspergillus can be isolated in the presence of counter-selective compounds, but the process is laborious. We developed a method to enable reversible impairment of the ku80 gene (Aaku80) in ...

    Authors: Shuji Tani, Atsushi Tsuji, Emi Kunitake, Jun-ichi Sumitani and Takashi Kawaguchi
    Citation: AMB Express 2013 3:4
  21. pH control has been essential for butanol production with Clostridium acetobutylicum. However, it is not very clear at what pH level the acid crash will occur, at what pH level butanol production will be dominant...

    Authors: Xuepeng Yang, Maobing Tu, Rui Xie, Sushil Adhikari and Zhaohui Tong
    Citation: AMB Express 2013 3:3
  22. A bacterial strain producing two antimicrobial peptides was isolated from a rhizosphere soil sample and identified as Bacillus subtilis based on both phenotypic and 16S rRNA gene sequence phylogenetic analysis. I...

    Authors: Piyush Baindara, Santi M Mandal, Niharika Chawla, Pradip Kumar Singh, Anil Kumar Pinnaka and Suresh Korpole
    Citation: AMB Express 2013 3:2
  23. Real-time PCR (qPCR) is a widely used technique in analysing environmental and clinical microbiological samples. However, its main limitation was its inability to discriminate between live and dead cells.

    Authors: Gerard Àlvarez, Marta González, Sergio Isabal, Vanessa Blanc and Rubén León
    Citation: AMB Express 2013 3:1
  24. Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monos...

    Authors: Violeta Sànchez i Nogué, Maurizio Bettiga and Marie F Gorwa-Grauslund
    Citation: AMB Express 2012 2:68
  25. Bacterial resistance against antibiotic treatment has become a major threat to public health. Antimicrobial peptides (AMPs) have emerged as promising alternative agents for treatment of infectious diseases. Th...

    Authors: Camilla Björn, Joakim Håkansson, Emma Myhrman, Veronika Sjöstrand, Tor Haug, Kerstin Lindgren, Hans-Matti Blencke, Klara Stensvåg and Margit Mahlapuu
    Citation: AMB Express 2012 2:67
  26. Intake of dietary fibres may reduce the prevalence of physiological risk factors of the metabolic syndrome, such as high plasma lipid levels and low-grade inflammatory state. Dietary fibres are usually of plan...

    Authors: Cecilia Lindström, Olle Holst, Lars Nilsson, Rickard Öste and Kristina E Andersson
    Citation: AMB Express 2012 2:66
  27. In this work we performed assays for the genetic improvement of the oleaginous yeast Lipomyces starkeyi DSM 70296 focusing on its utilization for lipid biosynthesis from renewable sources. The genetic optimizatio...

    Authors: Eulalia Tapia V, Andréia Anschau, Alessandro LV Coradini, Telma T Franco and Ana Carolina Deckmann
    Citation: AMB Express 2012 2:64
  28. The G-protein-coupled receptor (GPCR) superfamily, which includes somatostatin receptors (SSTRs), is one of the most important drug targets in the pharmaceutical industry. The yeast Saccharomyces cerevisiae is an...

    Authors: Keisuke Hara, Tomohiro Shigemori, Kouichi Kuroda and Mitsuyoshi Ueda
    Citation: AMB Express 2012 2:63
  29. In this study manganese peroxidase (MnP) enzymes from selected white-rot fungi were isolated and compared for potential future recombinant production. White-rot fungi were cultivated in small-scale in liquid m...

    Authors: Juho Järvinen, Sanna Taskila, Ritva Isomäki and Heikki Ojamo
    Citation: AMB Express 2012 2:62
  30. The goal of this study was to investigate the feasibility of bacterial cellulose (BC) scaffold to support osteoblast growth and bone formation. BC was produced by culturing Acetobacter xylinum supplemented with h...

    Authors: Neftaha Tazi, Ze Zhang, Younès Messaddeq, Luciana Almeida-Lopes, Lisinéia M Zanardi, Dennis Levinson and Mahmoud Rouabhia
    Citation: AMB Express 2012 2:61
  31. The analysis of microbial assemblages in industrial, marine, and medical systems can inform decisions regarding quality control or mitigation. Modern molecular approaches to detect, characterize, and quantify ...

    Authors: Athenia L Oldham, Heather S Drilling, Blake W Stamps, Bradley S Stevenson and Kathleen E Duncan
    Citation: AMB Express 2012 2:60
  32. Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery ...

    Authors: Daniel Heinrich, Mohamed H Madkour, Mansour A Al-Ghamdi, Ibraheem I Shabbaj and Alexander Steinbüchel
    Citation: AMB Express 2012 2:59
  33. To permit direct cellulose degradation and ethanol fermentation, Saccharomyces cerevisiae BY4741 (Δ sed1) codisplaying 3 cellulases (Trichoderma reesei endoglucanase II [EG], T. reesei cellobiohydrolase II [CBH],...

    Authors: Akihito Nakanishi, Jungu Bae, Kouichi Kuroda and Mitsuyoshi Ueda
    Citation: AMB Express 2012 2:56
  34. Like any other enteric pathogen, Salmonella also encounters acidic stress in the stomach as well as within the host macrophage milieu. However, the pathogen is reported to combat this stress through acid toleranc...

    Authors: Gaurav Jindal, Rupinder Tewari, Ankur Gautam, Satish K Pandey and Praveen Rishi
    Citation: AMB Express 2012 2:55
  35. The contaminant concentrations over which type strains of the species Dehalogenimonas alkenigignens and Dehalogenimonas lykanthroporepellens were able to reductively dechlorinate 1,2-dichloroethane (1,2-DCA), 1,2...

    Authors: Andrew D Maness, Kimberly S Bowman, Jun Yan, Fred A Rainey and William M Moe
    Citation: AMB Express 2012 2:54
  36. Enterococcus faecium No. 78 (PNCM-BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid in repeated batch fermentation mode. Enzymatically liquefied sago s...

    Authors: Cirilo Nolasco-Hipolito, Octavio Carvajal Zarrabal, Rubena Malfia Kamaldin, Ling Teck-Yee, Samuel Lihan, Kopli Bin Bujang and Youji Nitta
    Citation: AMB Express 2012 2:53
  37. Through metabolic engineering microorganisms can be engineered to produce new products and further produce these with higher yield and productivities. Here, we expressed the bacterial polyhydroxybutyrate (PHB)...

    Authors: Kanokarn Kocharin, Yun Chen, Verena Siewers and Jens Nielsen
    Citation: AMB Express 2012 2:52
  38. A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described. It is based on a multistage enzyme reaction which allows for the analysis of alkyl ha...

    Authors: Sebastian Fabritz, Franziska Maaß, Olga Avrutina, Tim Heiseler, Björn Steinmann and Harald Kolmar
    Citation: AMB Express 2012 2:51
  39. We recently established a method for isolating functional single cells from environmental samples using a micromanipulator (Functional single-cell (FSC) isolation), and applied it to the study of denitrifying ...

    Authors: Tomoyasu Nishizawa, Kanako Tago, Yusuke Uei, Satoshi Ishii, Kazuo Isobe, Shigeto Otsuka and Keishi Senoo
    Citation: AMB Express 2012 2:50
  40. Fusarium proliferatum NBRC109045 is a filamentous fungus isolated from Vietnamese forest due to high production of β-glucosidases. Production of the enzyme was studied on varied carbon source based mediums. The h...

    Authors: Ziqing Gao, Duong Van Hop, Le Thi Hoang Yen, Katsuhiko Ando, Shuichi Hiyamuta and Ryuichiro Kondo
    Citation: AMB Express 2012 2:49
  41. The biopreservation of foods using bacteriocinogenic lactic acid bacteria (LAB) isolated directly from foods is an innovative approach. The objectives of this study were to isolate and identify bacteriocinogen...

    Authors: En Yang, Lihua Fan, Yueming Jiang, Craig Doucette and Sherry Fillmore
    Citation: AMB Express 2012 2:48

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