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  1. A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions...

    Authors: Gaurav Singh Kaira, Kusum Dhakar and Anita Pandey
    Citation: AMB Express 2015 5:1
  2. Ethanol is by volume the largest fermentation product. During ethanol production by Saccharomyces cerevisiae about 4-5% of the carbon source is lost to glycerol production. Different approaches have been proposed...

    Authors: Clara Navarrete, Jens Nielsen and Verena Siewers
    Citation: AMB Express 2014 4:86
  3. L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicu...

    Authors: Dorit Eberhardt, Jaide V K Jensen and Volker F Wendisch
    Citation: AMB Express 2014 4:85
  4. Currently more than 90% of the world’s copper is obtained through sulfide mineral processing. Among the copper sulfides, chalcopyrite is the most abundant and therefore economically relevant. However, primary ...

    Authors: Roberto A Bobadilla-Fazzini, Maria Paz Cortés, Alejandro Maass and Pilar Parada
    Citation: AMB Express 2014 4:84
  5. The biosynthesis of poly(lactic acid) (PLA)-like polymers, composed of >99 mol% lactate and a trace amount of 3-hydroxybutyrate, in engineered Corynebacterium glutamicum consists of two steps; the generation of t...

    Authors: Ken’ichiro Matsumoto, Kota Tobitani, Shunsuke Aoki, Yuyang Song, Toshihiko Ooi and Seiichi Taguchi
    Citation: AMB Express 2014 4:83
  6. Affibody molecules specific for H-Ras and Raf-1 were evaluated for their ability to inhibit synovial cell function. Affibody molecules targeting H-Ras (Zras122, Zras220, and Zras521) or Raf-1 (Zraf322) were intro...

    Authors: Seiji Shibasaki, Miki Karasaki, Torbjörn Gräslund, Per-Åke Nygren, Hajime Sano and Tsuyoshi Iwasaki
    Citation: AMB Express 2014 4:82
  7. We established a protoplast-based system to transfer DNA to Knufia petricola strain A95, a melanised rock-inhabiting microcolonial fungus that is also a component of a model sub-aerial biofilm (SAB) system. To te...

    Authors: Steffi Noack-Schönmann, Tanja Bus, Ronald Banasiak, Nicole Knabe, William J Broughton, H Den Dulk-Ras, Paul JJ Hooykaas and Anna A Gorbushina
    Citation: AMB Express 2014 4:80
  8. The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conve...

    Authors: Domenico Schillaci, Maria Grazia Cusimano, Angelo Spinello, Giampaolo Barone, Debora Russo, Maria Vitale, Daniela Parrinello and Vincenzo Arizza
    Citation: AMB Express 2014 4:78
  9. A reporter system was constructed to measure perturbations in the NADH/NAD+ co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under con...

    Authors: Jan Dines Knudsen, Magnus Carlquist and Marie Gorwa-Grauslund
    Citation: AMB Express 2014 4:81
  10. NAD-dependent d-lactate dehydrogenases (d-LDHs) reduce pyruvate into d-lactate with oxidation of NADH into NAD+. Although non-allosteric d-LDHs from Lactobacilli have been extensively studied, the catalytic prope...

    Authors: Nayuta Furukawa, Akimasa Miyanaga, Misato Togawa, Masahiro Nakajima and Hayao Taguchi
    Citation: AMB Express 2014 4:76
  11. The blowout of the Deepwater Horizon in the Gulf of Mexico in 2010 occurred at a depth of 1500 m, corresponding to a hydrostatic pressure of 15 MPa. Up to now, knowledge about the impact of high pressure on oi...

    Authors: Martina Schedler, Robert Hiessl, Ana Gabriela Valladares Juárez, Giselher Gust and Rudolf Müller
    Citation: AMB Express 2014 4:77
  12. Several species of white-rot fungi were investigated for their utility in prolonged decolouration of the recalcitrant sulfonated azo dye, amaranth. Trametes pubescens, T. multicolor, T. meyenii and T. versicolor ...

    Authors: Peter R Chenaux, Narisa Lalji and Daniel D Lefebvre
    Citation: AMB Express 2014 4:74
  13. Rhodococcus opacus R7 is a Gram-positive bacterium isolated from a polycyclic aromatic hydrocarbon contaminated soil for its versatile metabolism; indeed the strain is able to grow on naphthalene, o-xylene, and s...

    Authors: Jessica Zampolli, Elena Collina, Marina Lasagni and Patrizia Di Gennaro
    Citation: AMB Express 2014 4:73
  14. Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid residues, exhibit only seven amino acid residue differences between them, but LinBMI’s catalytic performance towards β-hexachlorocyclohexa...

    Authors: Ryota Moriuchi, Hiroki Tanaka, Yuki Nikawadori, Mayuko Ishitsuka, Michihiro Ito, Yoshiyuki Ohtsubo, Masataka Tsuda, Jiri Damborsky, Zbynek Prokop and Yuji Nagata
    Citation: AMB Express 2014 4:72
  15. Viable bacterial cells impaled with a single particle of a nano-sized acicular material formed when a mixture containing the cells and the material was exposed to a sliding friction field between polystyrene a...

    Authors: Yuya Mitsudome, Mamiko Takahama, Jun Hirose and Naoto Yoshida
    Citation: AMB Express 2014 4:70
  16. Microbial biotechnology and biotransformations promise to diversify the scope of the biorefinery approach for the production of high-value products and biofuels from industrial, rural and municipal waste feeds...

    Authors: Claire M Hull, E Joel Loveridge, Iain S Donnison, Diane E Kelly and Steven L Kelly
    Citation: AMB Express 2014 4:64
  17. β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. In this study we report the heterologous expression of Aspergillus oryzae β-xylosidase (XylA) in...

    Authors: Narin Kirikyali, Jonathan Wood and Ian F Connerton
    Citation: AMB Express 2014 4:68
  18. Higher initial glycerol loadings (620 mM) have a negative effect on growth and 1,3-propanediol (1,3-PDO) synthesis in Clostridium butyricum DSM 10702 relative to lower initial glycerol concentrations (170 mM). To...

    Authors: Mine Gungormusler-Yilmaz, Dmitry Shamshurin, Marine Grigoryan, Marcel Taillefer, Victor Spicer, Oleg V Krokhin, Richard Sparling and David B Levin
    Citation: AMB Express 2014 4:63
  19. Population heterogeneity occurring in industrial microbial bioprocesses is regarded as a putative effector causing performance loss in large scale. While the existence of subpopulations is a commonly accepted ...

    Authors: Sarah Lieder, Michael Jahn, Jana Seifert, Martin von Bergen, Susann Müller and Ralf Takors
    Citation: AMB Express 2014 4:71
  20. In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB’, gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been heterologously ex...

    Authors: Elena Volodina and Alexander Steinbüchel
    Citation: AMB Express 2014 4:69
  21. Elucidation of the mechanism of high temperature tolerance in yeasts is important for the molecular breeding of high temperature-tolerant yeasts that can be used in bioethanol production. We identified genes w...

    Authors: Toshihide Nakamura, Mami Yamamoto, Katsuichi Saito, Akira Ando and Jun Shima
    Citation: AMB Express 2014 4:67
  22. Pectin is a structural heteropolysaccharide of the primary cell walls of plants and as such is a significant fraction of agricultural waste residues that is currently insufficiently used. Its main component, D...

    Authors: Jasper Sloothaak, Mike Schilders, Peter J Schaap and Leo H de Graaff
    Citation: AMB Express 2014 4:66
  23. Aspergillus sp. contain ppo genes coding for Ppo enzymes that produce oxylipins from polyunsaturated fatty acids. These oxylipins function as signal molecules in sporulation and influence the asexual to sexual ra...

    Authors: Ruud Heshof, J Paul van Schayck, Juan Antonio Tamayo-Ramos and Leo H de Graaff
    Citation: AMB Express 2014 4:65
  24. The proteinase K subfamily enzymes, thermophilic Aqualysin I (AQN) from Thermus aquaticus YT-1 and psychrophilic serine protease (VPR) from Vibrio sp. PA-44, have six and seven salt bridges, respectively. To unde...

    Authors: Masayoshi Sakaguchi, Kanae Osaku, Susumu Maejima, Nao Ohno, Yasusato Sugahara, Fumitaka Oyama and Masao Kawakita
    Citation: AMB Express 2014 4:59
  25. Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic reco...

    Authors: Alma Lorena Almaraz-Delgado, José Flores-Uribe, Víctor Hugo Pérez-España, Edgar Salgado-Manjarrez and Jesús Agustín Badillo-Corona
    Citation: AMB Express 2014 4:57
  26. Enriched cultures of microorganisms are an essential step in the production of inoculum of these organisms for biotechnology and bioengineering. The potential application of methanotrophic microorganisms for r...

    Authors: Zofia Stępniewska and Agnieszka Kuźniar
    Citation: AMB Express 2014 4:58
  27. Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, ...

    Authors: Valeria Wallace-Salinas, Lorenzo Signori, Ying-Ying Li, Magnus Ask, Maurizio Bettiga, Danilo Porro, Johan M Thevelein, Paola Branduardi, María R Foulquié-Moreno and Marie Gorwa-Grauslund
    Citation: AMB Express 2014 4:56
  28. Fungal volatile organic compounds (VOCs) play important ecophysiological roles in mediating inter-kingdom signaling with arthropods but less is known about their interactions with plants. In this study, Arabidops...

    Authors: Richard Hung, Samantha Lee, Cesar Rodriguez-Saona and Joan W Bennett
    Citation: AMB Express 2014 4:53
  29. Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extra...

    Authors: Licia Lama, Annabella Tramice, Ilaria Finore, Gianluca Anzelmo, Valeria Calandrelli, Eduardo Pagnotta, Giuseppina Tommonaro, Annarita Poli, Paola Di Donato, Barbara Nicolaus, Massimo Fagnano, Mauro Mori, Adriana Impagliazzo and Antonio Trincone
    Citation: AMB Express 2014 4:55
  30. Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. Increasing interest in controlling water-borne pathogens in water resources ev...

    Authors: Pramod K Pandey, Philip H Kass, Michelle L Soupir, Sagor Biswas and Vijay P Singh
    Citation: AMB Express 2014 4:51
  31. Nowadays antimicrobial lipopeptides are being widely exploited for developing potential therapeutic agents for treating bacterial infections. In the present study, we have purified and characterized an antimic...

    Authors: Deepika Sharma, Santi M Mandal and Rajesh Kumari Manhas
    Citation: AMB Express 2014 4:50
  32. Anhydromevalonolactone (AMVL) is a bioactive natural product that arises from a molecular biology technique using Aspergillus oryzae as a heterologous host. AMVL has been used as a precursor for the synthesis of ...

    Authors: Songsak Wattanachaisaereekul, Anuwat Tachaleat, Juntira Punya, Rachada Haritakun, Chollaratt Boonlarppradab and Supapon Cheevadhanarak
    Citation: AMB Express 2014 4:52
  33. The suitability of paper-based arrays for biofilm formation studies by Staphylococcus aureus is demonstrated. Laboratory-coated papers with different physicochemical properties were used as substrates. The array ...

    Authors: Anni Määttänen, Adyary Fallarero, Janni Kujala, Petri Ihalainen, Pia Vuorela and Jouko Peltonen
    Citation: AMB Express 2014 4:32
  34. Biofilms in the industrial environment could be problematic. Encased in extracellular polymeric substances, pathogens within biofilms are significantly more resistant to chlorine and other disinfectants. Recen...

    Authors: Massimiliano Marvasi, Charles Chen, Manuel Carrazana, Ian A Durie and Max Teplitski
    Citation: AMB Express 2014 4:42
  35. Thermomonospora curvata is a thermophilic actinomycete phylogenetically related to Thermobifida fusca that produces extracellular hydrolases capable of degrading synthetic polyesters. Analysis of the genome of T....

    Authors: Ren Wei, Thorsten Oeser, Johannes Then, Nancy Kühn, Markus Barth, Juliane Schmidt and Wolfgang Zimmermann
    Citation: AMB Express 2014 4:44
  36. We investigated the severity of the inhibitory effects of 13 phenolic compounds usually found in spruce hydrolysates (4-hydroxy-3-methoxycinnamaldehyde, homovanilyl alcohol, vanillin, syringic acid, vanillic a...

    Authors: Peter Temitope Adeboye, Maurizio Bettiga and Lisbeth Olsson
    Citation: AMB Express 2014 4:46
  37. Trichoderma spp., a known beneficial fungus is reported to have several mechanisms to enhance plant growth. In this study, the effectiveness of seven isolates of Trichoderma spp. to promote growth and increase ph...

    Authors: Febri Doni, Anizan Isahak, Che Radziah Che Mohd Zain and Wan Mohtar Wan Yusoff
    Citation: AMB Express 2014 4:45
  38. Agrobacterium tumefaciens-mediated transformation is a powerful tool for reverse genetics and functional genomic analysis in a wide variety of plants and fungi. Tuber spp. are ecologically important and gastronom...

    Authors: Andrea Brenna, Barbara Montanini, Eleonora Muggiano, Marco Proietto, Patrizia Filetici, Simone Ottonello and Paola Ballario
    Citation: AMB Express 2014 4:43
  39. We overexpressed the err1 gene in the Trichoderma reesei wild-type and in the cellulase hyperproducing, carbon catabolite derepressed strain Rut-C30 in order to investigate the possibility of producing erythritol...

    Authors: Birgit Jovanović, Robert L Mach and Astrid R Mach-Aigner
    Citation: AMB Express 2014 4:34
  40. A novel strain of Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize medium chain-length polyhydroxyalkanoates (mcl-PHAs). P.putida LS46 was differentiated from other P...

    Authors: Parveen K Sharma, Jilagamazhi Fu, Xiangli Zhang, Brian Fristensky, Richard Sparling and David B Levin
    Citation: AMB Express 2014 4:37
  41. Currently, pursuing yeast strains that display both a high potential fitness for alcoholic fermentation and a favorable impact on quality is a major goal in the alcoholic beverage industry. This considerable i...

    Authors: Catarina Barbosa, Patrícia Lage, Alice Vilela, Arlete Mendes-Faia and Ana Mendes-Ferreira
    Citation: AMB Express 2014 4:39

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