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  1. Content type: Original article

    To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain is...

    Authors: Motoo Koitabashi, Masako T Noguchi, Yuka Sameshima-Yamashita, Syuntaro Hiradate, Ken Suzuki, Shigenobu Yoshida, Takashi Watanabe, Yukiko Shinozaki, Seiya Tsushima and Hiroko K Kitamoto

    Citation: AMB Express 2012 2:40

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  2. Content type: Original article

    The aminopeptidase P (PepP, EC 3.4.11.9) gene from Lactococcus lactis ssp. lactis DSM 20481 was cloned, sequenced and expressed recombinantly in E. coli BL21 (DE3) for the first time. PepP is involved in the hydr...

    Authors: Timo Stressler, Thomas Eisele, Michael Schlayer and Lutz Fischer

    Citation: AMB Express 2012 2:39

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  3. Content type: Original article

    Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work ...

    Authors: Juan A Vallejo, Manuel Serrat, Irasema Pérez-Portuondo, Angeles Sánchez-Pérez, Jose M Ageitos and Tomas G Villa

    Citation: AMB Express 2012 2:38

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  4. Content type: Original article

    We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long mono...

    Authors: Hironobu Morisaka, Kazuma Matsui, Yohei Tatsukami, Kouichi Kuroda, Hideo Miyake, Yutaka Tamaru and Mitsuyoshi Ueda

    Citation: AMB Express 2012 2:37

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  5. Content type: Original article

    Single cell oils (SCOs) accumulated by oleaginous yeasts have emerged as potential alternative feedstocks for biodiesel production. As lipid accumulation is species and substrate specific, selection of an appr...

    Authors: Gouri Katre, Chirantan Joshi, Mahesh Khot, Smita Zinjarde and Ameeta RaviKumar

    Citation: AMB Express 2012 2:36

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  6. Content type: Original article

    Vinasse, a highly polluting waste of the ethanol industry was utilized for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloferax mediterranei in shake-flasks. Following pre-...

    Authors: Anirban Bhattacharyya, Arnab Pramanik, Sudipta Kumar Maji, Saubhik Haldar, Ujjal Kumar Mukhopadhyay and Joydeep Mukherjee

    Citation: AMB Express 2012 2:34

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  7. Content type: Original article

    S-hydroxymethylglutathione dehydrogenase from Paecilomyces variotii No. 5 strain (NBRC 109023), isolated as a formaldehyde-degrading fungus, was purified by a procedure that included ammonium sulfate precipitatio...

    Authors: Ryohei Fukuda, Kazuhiro Nagahama, Kohsai Fukuda, Keisuke Ekino, Takuji Oka and Yoshiyuki Nomura

    Citation: AMB Express 2012 2:32

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  8. Content type: Original article

    We isolated a mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp. ATCC 31750 by chemical mutagenesis using N-methyl-N-nitro-nitrosoguanidine. The mutant strain produced 66 g/L of ...

    Authors: Gayathiri T Kalyanasundaram, Mukesh Doble and Sathyanarayana N Gummadi

    Citation: AMB Express 2012 2:31

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  9. Content type: Original article

    Aerobic microbial degradation of p-nitrophenol (PNP) has been classically shown to proceed via ‘Hydroquinone (HQ) pathway’ in Gram-negative bacteria, whereas in Gram-positive PNP degraders it proceed via ‘Benzene...

    Authors: Surendra Vikram, Janmejay Pandey, Nidhi Bhalla, Gunjan Pandey, Anuradha Ghosh, Fazlurrahman Khan, Rakesh K Jain and Gajendra PS Raghava

    Citation: AMB Express 2012 2:30

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  10. Content type: Original article

    We purified and cloned a β-N-acetylhexosaminidase, LeHex20A, with a molecular mass of 79 kDa from the fruiting body of Lentinula edodes (shiitake mushroom). The gene lehex20a gene had 1,659 nucleotides, encoding ...

    Authors: Naotake Konno, Hideyuki Takahashi, Masahiro Nakajima, Takumi Takeda and Yuichi Sakamoto

    Citation: AMB Express 2012 2:29

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  11. Content type: Original article

    Production of the Alternaria mycotoxins alternariol (AOH), alternariol monomethylether (AME) and tenuazonic acid (TA) by Alternaria alternata DSM 12633 was influenced by pH and carbon to nitrogen (C:N) ratio of t...

    Authors: Katrin Brzonkalik, Dominik Hümmer, Christoph Syldatk and Anke Neumann

    Citation: AMB Express 2012 2:28

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  12. Content type: Original article

    In this study, a novel rDNA based plasmid was developed for display of heterologous proteins on the cell surface of Yarrowia lipolytica using the C-terminal end of the glycosylphosphatidylinositol (GPI) anchored

    Authors: Siyavuya Ishmael Bulani, Lucy Moleleki, Jacobus Albertyn and Ntsane Moleleki

    Citation: AMB Express 2012 2:27

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  13. Content type: Original article

    Polyhydroxyalkanoates (PHA) are biodegradable polymers that are attractive materials for use in tissue engineering and medical device manufacturing. Ralstonia eutropha is regarded as the model organism for PHA bi...

    Authors: Christopher J Brigham, Esther N Reimer, ChoKyun Rha and Anthony J Sinskey

    Citation: AMB Express 2012 2:26

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  14. Content type: Original article

    In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a...

    Authors: José Miguel Araya-Garay, José M Ageitos, Juan A Vallejo, Patricia Veiga-Crespo, Angeles Sánchez-Pérez and Tomás G Villa

    Citation: AMB Express 2012 2:24

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  15. Content type: Original article

    Volatile hydrocarbon production by Ascocoryne sacroides was studied over its growth cycle. Gas-phase compounds were measured continuously with a proton transfer reaction-mass spectrometry (PTR-MS) and at distinct...

    Authors: Natasha D Mallette, W Berk Knighton, Gary A Strobel, Ross P Carlson and Brent M Peyton

    Citation: AMB Express 2012 2:23

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  16. Content type: Original article

    Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP)....

    Authors: Claudia P Martínez, Claudia Echeverri, Juan C Florez, Alvaro L Gaitan and Carmenza E Góngora

    Citation: AMB Express 2012 2:22

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  17. Content type: Original article

    Lactobacillus plantarum is involved in a multitude of food related industrial fermentation processes including the malolactic fermentation (MLF) of wine. This work is the first report on a recombinant L. plantaru...

    Authors: Christina Schümann, Herbert Michlmayr, Reinhard Eder, Andrés M del Hierro, Klaus D Kulbe, Geir Mathiesen and Thu-Ha Nguyen

    Citation: AMB Express 2012 2:19

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  18. Content type: Original article

    In petrochemical refinery wastewater treatment plants (WWTP), different concentrations of pollutant compounds are received daily in the influent stream, including significant amounts of phenolic compounds, cre...

    Authors: Cynthia C Silva, Helen Hayden, Tim Sawbridge, Pauline Mele, Ricardo H Kruger, Marili VN Rodrigues, Gustavo GL Costa, Ramon O Vidal, Maíra P Sousa, Ana Paula R Torres, Vânia MJ Santiago and Valéria M Oliveira

    Citation: AMB Express 2012 2:18

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  19. Content type: Original article

    Plate assays for lipoxygenase producing microorganisms on agar plates have been developed. Both potassium iodide-starch and indamine dye formation methods were effective for detecting soybean lipoxygenase acti...

    Authors: Antti Nyyssölä, Ruud Heshof, Thomas Haarmann, Jasmin Eidner, Ann Westerholm-Parvinen, Kim Langfelder, Kristiina Kruus, Leo de Graaff and Johanna Buchert

    Citation: AMB Express 2012 2:17

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  20. Content type: Original article

    Microbial bioprocessing of lignocellulose to bioethanol still poses challenges in terms of substrate catabolism. A targeted evolution-based study was undertaken to determine if inter-strain microbial variabili...

    Authors: Shahin S Ali, Mojibur Khan, Brian Fagan, Ewen Mullins and Fiona M Doohan

    Citation: AMB Express 2012 2:16

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  21. Content type: Original

    Bikunin is a proteoglycan exhibiting broad-spectrum inhibitory activity against serine proteases and could potentially suppress tumor cell invasion and metastasis. Here, we have successfully expressed recombin...

    Authors: Xing-Hua Gou, Yu-Ying Liu, Qi-Lei Chen, Jian-Jun Tang, Da-Yu Liu, Liang Zou, Xiao-Yong Wu and Wei Wang

    Citation: AMB Express 2012 2:14

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  22. Content type: Original

    Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabino...

    Authors: Bala Muntari, Azura Amid, Maizirwan Mel, Mohammed S Jami and Hamzah M Salleh

    Citation: AMB Express 2012 2:12

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  23. Content type: Mini-Review

    Optically pure β-amino acids constitute interesting building blocks for peptidomimetics and a great variety of pharmaceutically important compounds. Their efficient synthesis still poses a major challenge. Tra...

    Authors: Jens Rudat, Birgit R Brucher and Christoph Syldatk

    Citation: AMB Express 2012 2:11

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  24. Content type: Original

    To attempt cost-effective production of US417 phytase in Bacillus subtilis, we developed an efficient system for its large-scale production in the generally recognized as safe microorganism B. subtilis 168. Hence...

    Authors: Ameny Farhat-Khemakhem, Mounira Ben Farhat, Ines Boukhris, Wacim Bejar, Kameleddine Bouchaala, Radhouane Kammoun, Emmanuelle Maguin, Samir Bejar and Hichem Chouayekh

    Citation: AMB Express 2012 2:10

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  25. Content type: Original

    Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed ba...

    Authors: Ryoich Nakamura, Ryuichiro Kondo, Ming-hao Shen, Hideharu Ochiai, Shin Hisamatsu and Shigenori Sonoki

    Citation: AMB Express 2012 2:8

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  26. Content type: Original

    The capacity of white-rot fungi to degrade wood lignin may be highly applicable to the development of novel bioreactor systems, but the mechanisms underlying this function are not yet fully understood. Lignin ...

    Authors: Takaiku Sakamoto, Yuki Yao, Yoshifumi Hida, Yoichi Honda, Takashi Watanabe, Wataru Hashigaya, Kazumi Suzuki and Toshikazu Irie

    Citation: AMB Express 2012 2:7

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  27. Content type: Original

    Large volumes of treated process water are required for protein extraction. Evaporation of this water contributes greatly to the energy consumed in enriching protein products. Thin stillage remaining from etha...

    Authors: Kornsulee Ratanapariyanuch, Robert T Tyler, Youn Young Shim and Martin JT Reaney

    Citation: AMB Express 2012 2:5

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  28. Content type: Original

    The gene for a eukaryotic phenolic acid decarboxylase of Candida guilliermondii was cloned, sequenced, and expressed in Escherichia coli for the first time. The structural gene contained an open reading frame of ...

    Authors: Hui-Kai Huang, Li-Fan Chen, Masamichi Tokashiki, Tadahiro Ozawa, Toki Taira and Susumu Ito

    Citation: AMB Express 2012 2:4

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  29. Content type: Original

    Analysis of the simocyclinone biosynthesis (sim) gene cluster of Streptomyces antibioticus Tü6040 led to the identification of a putative pathway specific regulatory gene simReg1. In silico analysis places the Si...

    Authors: Liliya Horbal, Yuriy Rebets, Mariya Rabyk, Roman Makitrynskyy, Andriy Luzhetskyy, Victor Fedorenko and Andreas Bechthold

    Citation: AMB Express 2012 2:1

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  30. Content type: Original

    Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence fr...

    Authors: Georgiana F da Cruz, Suzan P de Vasconcellos, Célio FF Angolini, Bruna M Dellagnezze, Isabel NS Garcia, Valéria M de Oliveira, Eugenio V dos Santos Neto and Anita J Marsaioli

    Citation: AMB Express 2011 1:47

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  31. Content type: Original

    Agrobacterium tumefaciens-mediated transformation (AMT) was applied to Aspergillus aculeatus. Transformants carrying the T-DNA from a binary vector pBIG2RHPH2 were sufficiently mitotically stable to allow functi...

    Authors: Emi Kunitake, Shuji Tani, Jun-ichi Sumitani and Takashi Kawaguchi

    Citation: AMB Express 2011 1:46

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  32. Content type: Mini-Review

    Systems Biology has taken advantage of computational tools and high-throughput experimental data to model several biological processes. These include signaling, gene regulatory, and metabolic networks. However...

    Authors: Daniel Machado, Rafael S Costa, Miguel Rocha, Eugénio C Ferreira, Bruce Tidor and Isabel Rocha

    Citation: AMB Express 2011 1:45

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  33. Content type: Original

    The use of biodegradable plastics can reduce the accumulation of environmentally persistent plastic wastes. The rate of degradation of biodegradable plastics depends on environmental conditions and is highly v...

    Authors: Hiroko K Kitamoto, Yukiko Shinozaki, Xiao-hong Cao, Tomotake Morita, Masaaki Konishi, Kanako Tago, Hideyuki Kajiwara, Motoo Koitabashi, Shigenobu Yoshida, Takashi Watanabe, Yuka Sameshima-Yamashita, Toshiaki Nakajima-Kambe and Seiya Tsushima

    Citation: AMB Express 2011 1:44

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  34. Content type: Original

    We screened various thermophiles for meso-diaminopimelate dehydrogenase (meso-DAPDH, EC 1.4.1.16), which catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate, and found the enzyme in a the...

    Authors: Hironaga Akita, Yasuhiro Fujino, Katsumi Doi and Toshihisa Ohshima

    Citation: AMB Express 2011 1:43

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  35. Content type: Original

    Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay ...

    Authors: Asha A Kulkarni-Almeida, Manoja K Brahma, Prabhu Padmanabhan, Prabhu D Mishra, Rajashri R Parab, Nitin V Gaikwad, Chandni S Thakkar, Pradipta Tokdar, Prafull V Ranadive, Amrutha S Nair, Anagha A Damre, Umakant A Bahirat, Nitin J Deshmukh, Lalit S Doshi, Amol V Dixit, Saji D George…

    Citation: AMB Express 2011 1:42

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  36. Content type: Original

    The microbes in the gastrointestinal (GI) tract are of high importance for the health of the host. In this study, Roche 454 pyrosequencing was applied to a pooled set of different 16S rRNA gene amplicons obtai...

    Authors: Maartje AHJ van Kessel, Bas E Dutilh, Kornelia Neveling, Michael P Kwint, Joris A Veltman, Gert Flik, Mike SM Jetten, Peter HM Klaren and Huub JM Op den Camp

    Citation: AMB Express 2011 1:41

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  37. Content type: Original

    A major problem for manufacturers of cracked spores Ganoderma lucidum, a traditional functional food/Chinese medicine (TCM), is to ensure that raw materials are consistent as received from the producer. To addres...

    Authors: Chee Wei Lim, Sheot Harn Chan and Angelo Visconti

    Citation: AMB Express 2011 1:40

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  38. Content type: Original

    With the emergence and spread of multidrug resistant bacteria, effective methods to eliminate both planktonic bacteria and those embedded in surface-attached biofilms are needed. Electric currents at μA-mA/cm2 ra...

    Authors: Robert Szkotak, Tagbo H R Niepa, Nikhil Jawrani, Jeremy L Gilbert, Marcus B Jones and Dacheng Ren

    Citation: AMB Express 2011 1:39

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