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Table 4 Sensitivities and specificities of the two amplification systems of rpoB –based PCR sequencing and Real-time PCR (N = 124)

From: Molecular identification of non-tuberculous mycobacterial species isolated from extrapulmonary samples using real-time PCR and rpoB sequence analysis

Type of sample and Method

Samples no.

Prevalence

95% CIa

Positive LRb

Negative LR

lymph node (N = 54)

     

rpoB sequencing

39

72.22%

79–95

72.95

0.90

real-time PCR

33

61.11%

46–59

60.01

0.82

urine(N = 20)

     

rpoB sequencing

3

0.15%

73–95

73.94

0.91

real-time PCR

1

0.05%

28–45

14.24

0.79

skin lesion (N = 10)

     

rpoB sequencing

10

100%

70–95

100

0.00

real-time PCR

10

100%

70–95

100

0.00

pleural fluid (N = 34)

     

rpoB sequencing

23

67.64%

69–95

79.97

0.95

real-time PCR

23

67.64%

33–54

79.27

0.95

bone biopsy(N = 6)

     

rpoB sequencing

2

68.79%

62–95

79.95

0.99

real-time PCR

-

-

-

-

-

  1. CLa: confidence interval, LRb: likelihood ratios