Extracellular production of a thermostable Cellvibrio endolytic β-agarase in Escherichia coli for agarose liquefaction

AMB Express

Table 1 Effect of metal ions and other chemicals on GH16B β-agarase activity

Metal ion/chemical (mM)		Relative activity (%)^a	Metal ion/chemical (mM)		Relative activity (%)^a
Control		100 (± 1.15)	CaCl₂	0.5	96 (± 1.80)
				1.0	110 (± 6.85)
				2.5	103 (± 0.90)
MnCl₂	0.5	125 (± 1.40)	KCl	0.5	103 (± 1.20)
	1.0	163 (± 2.60)		1.0	109 (± 6.41)
	2.5	155 (± 3.00)		2.5	104 (± 3.45)
MnSO₄	0.5	146 (± 1.95)	CuSO₄	0.5	103 (± 0.25)
	1.0	156 (± 0.70)		1.0	97 (± 1.45)
	2.5	156 (± 1.90)		2.5	74 (± 1.45)
MgCl₂	0.5	94 (± 2.80)	TCEP	0.5	100 (± 1.55)
	1.0	107 (± 0.80)		1.0	109 (± 2.85)
	2.5	100 (± 3.55)		2.5	117 (± 6.00)
NaCl	0.5	100 (± 0.30)	EDTA	0.5	95 (± 4.75)
	1.0	109 (± 5.33)		1.0	95 (± 2.02)
	2.5	101 (± 2.25)		2.5	91 (± 4.35)

^aValues represent the agarase activity (%) expressed as mean ± SD (n = 3) relative to nontreated control