Fig. 8

Analysis of GH16B β-agarase-catalyzed hydrolysates of agarose and AOS using MALDI–TOF/TOF MS. a For agarose hydrolysis, 0.4%[w/v] agarose was treated with the purified enzyme (0.8 µg/mL) in 50 mM Mcllvaine buffer (pH 7.0) at 50 °C for 60 min. b For AOS hydrolysis, 1.0%[w/v] AOS was treated with the purified enzyme (0.8 µg/mL) in 50 mM Mcllvaine buffer (pH 7.0) at 50 °C for 60 min. MALDI–TOF/TOF MS analysis was performed as described in “Materials and methods” section