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AMB Express

Table 3 Lactate dehydrogenase (LDH), protease and acetylcholinesterase (AchE) activities of the laboratory S. littoralis treated with Streptomyces sp. ES2 crude metabolite

From: Endophytic actinobacteria from wild medicinal plants are a natural source of insecticide to control the African cotton leafworm (Spodoptera littoralis)

Treatment

LDH (U × 103/mg protein)

Protease (ug D,L-alanine/min/mg protein)

AchE (ug AchBr/min/mg protein)

Normal distilled water

28.4 ± 0.89 a

101.66 ± 3.84 a

2.40 ± 0.09 a

Negative Control Ethyl acetate

26.42 ± 0.68 a

92.33 ± 1.45 b

2.15 ± 0.09 a

Radiant SC 12% (0.05%)

23.8 ± 0.62 b

30.33 ± 0.88 c

2.31 ± 0.06 a

ES2 Metabolite (100 mg/mL)

15.06 ± 0.54 c

37.00 ± 2.08 c

2.16 ± 0.05 a

ANOVA- 1 way

 F-ratio

72.25

247.08

2.69

 p-value

 < 0.001***

0.001***

 > 0.05 NS

  1. Data expressed as Mean ± S. E Mean. Different letters (a, b & c) in the same column denote a significant different between groups, according to DMRTs, at p ≤ 0.05. *** = p ≤ 0.01 NS = non-Significant
  2. Normal, Control treated with distilled water for experiment adjustment; Negative Control, control treated with ethyl acetate solvent for experiment adjustment; ES2 Metabolite, the most potent actinobacterial crude metabolite produced by Streptomyces sp. ES2 strain; F-ratio, Frequency ratio
  3. All biochemical measurements were performed in triplicates
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