An improved integrative GFP-based vector for genetic engineering of Parageobacillus thermoglucosidasius facilitates the identification of a key sporulation regulator

AMB Express

Table 1 Strains and plasmids used in the present study

Strain	Relevant genotype or properties	Source
P. thermoglucosidasius DSM2542/BGSC ID 95A1	Wildtype isolate	Bacillus Genetic Stock Center (USA)
Pth::pMM7	DSM2542::pMM7 Kan^R	This work
Pth ∆spo0A	DSM2542 Δspo0A	This work
E. coli DH5α™	F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk +) phoA supE44 thi-1 gyrA96 relA1 λ-	ThermoFisher Scientific
E. coli DH5α-λpir	F- φ80lacZ∆M15 ∆(lacZYA-argF)U169 recA1 endA1 hsdR17(rk-, mk +) phoA supE44 thi-1 gyrA96 relA1 λ-pir lysogen of DH5α	Lab collection

Plasmid	Description	Source
pMTL61110	E. coli/P. thermoglucosidasius integrative shuttle vector, Thermosensitive replicon	Sheng et al. 2017
pIP7	Vector carrying sfGFP	Pogrebnyakov et al. 2017
pGB-sfGFP	pMTL61110 carrying P7-sfGFP	This work
pGB-sfGFP-best	pGB-sfGFP (F11V, T13P, N39D, A179A)	This work
pMM7	pGB-sfGFP-best carrying flanking regions of spo0A	This work